文献类型：期刊文献

中文题名：酸碱度和松树基质对杀线菌Esteya vermicola菌丝体生长的影响

英文题名：Effects of pH Value and Pine Substrate on Growth of Esteya vermicola Mycelia

作者：王瑞珍[1,2] 温韦华[2] 刘浡洋[2] 崔夏[2] 贺然[1,2] 曲良建[1] 张永安[1,3]

第一作者：王瑞珍

机构：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业和草原局森林保护学重点实验室,北京100091;[2]北京市植物园/北京市花卉园艺工程技术研究中心,北京100093;[3]中国林业科学研究院华北林业实验中心,北京100000

年份：2020

卷号：33

期号：3

起止页码：139-145

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家重点研发计划项目(2018YFC1200400);国家自然科学基金项目(31800547)。

语种：中文

中文关键词：黑松;Esteya vermicola;松材线虫;生物防治

外文关键词：Pinus thunbergii;Esteya vermicola;Bursaphelenchus xylophilus;Biological control

分类号：S763

摘要：[目的]研究Esteya vermicola真菌适应的pH范围,并探讨松树成分对其生长的影响,以期为E.vermicola菌在松树体内的定植和林业重大病害松材线虫野外的生物防治提供理论支撑。[方法]选取E.vermicola CBS115803(EV115803)和E.vermicola CNU120806(EV120806)为试验对象,测试EV真菌适应的pH范围,并以35 a黑松不同器官组织配制培养基,探究EV菌的生长情况,观测各菌株生长速率、菌体量和产孢量。[结果]EV菌适应的pH值范围为5~13,菌体量在酸性条件下产生更多的气生菌丝。PDA中加入松树成分对EV菌有一定的刺激生长作用。在以松针、松树皮、松枝条为唯一营养来源时,EV菌生长茂盛。以木质部锯末为单一营养源时,EV菌生长缓慢。[结论]EV菌适应的pH值范围为5~13,EV菌耐碱性极强,且在加有松树各部位成分的培养基上生长,菌株EV120806对松树培养基的适应性更强,未来有望将EV菌定殖在松树中做为共生菌,摄食入侵的松材线虫,从而使松树具有内源的防控因子防控松材线虫。
[Objective]To study the pH range favoring Esteya vermicola,and the effects of pine substrate on the growth of E.vermicola so as to provide references for colonization of E.vermicola in Pinus trees and the biological control against pine wood nematodes.[Method]E.vermicola CBS115803(EV115803)and E.vermicola CNU120806(EV120806)were used to test their fungal pH adaptation range.The media with different parts of pine were prepared to study the growth of E.vermicola and to observe and measure the growth rate,mycelial amount and sporulation of each strain.[Result]E.vermicola was adapted to the pH range of 5 to 13,and the fungi produced more aerial hyphae under acidic conditions.PDA medium added with different parts of pine trees had a certain stimulating effect for E.vermicola.When pine needles,pine bark,and pine twigs were used as the only nutrient source,E.vermicola grew better than on WA plate.E.vermicola strains grew slowly when xylem sawdust was used as a single nutrient source.[Conclusion]E.vermicola is adapted to the pH range of 5 to 13.It is extremely resistant to alkaline and can grow on media added with different parts of pine trees.It is expected that E.vermicola will colonize the pine trees as symbiotic fungi against invading pine wood nematodes,so that pine trees have endogenous control factors for pine wood nematodes.