文献类型：期刊文献

英文题名：Overexpression of a gibberellin 20-oxidase gene in poplar xylem led to an increase in the size of nanocellulose fibrils and improved paper properties

作者：Peng, Xiaopeng[1,2] Tong, Botong[1,3] Lee, Jongcheol[4] Wang, Kun[5] Yu, Xiaojuan[5] Huang, Xiong[1] Wen, Jialong[5] Makarem, Mohamadamin[4] Pang, Hongying[1] Hinjan, Subin[6] Yan, Xiaojing[1] Yao, Shuangquan[7] Lu, Fachuang[8,9] Wang, Baichen[10] Peng, Feng[5] Ralph, John[8,9] Kim, Seong H.[4] Sederoff, Ronald R.[3,11] Li, Quanzi[1]

第一作者：彭宵鹏

通信作者：Li, QZ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[4]Penn State Univ, Mat Res Inst, Dept Chem Engn, University Pk, PA 16802 USA;[5]Beijing Forestry Univ, Coll Mat Sci & Technol, Beijing 100083, Peoples R China;[6]Bangkok Cordyceps Co Ltd, Thanyaburi 12130, Pathumthani, Thailand;[7]Guangxi Univ, Coll Light Ind & Food Engn, Guangxi Key Lab Clean Pulp & Papermaking & Pollut, Nanning 530004, Peoples R China;[8]Univ Wisconsin, Wisconsin Energy Inst, Dept Biochem, Madison, WI 53726 USA;[9]Univ Wisconsin, Wisconsin Energy Inst, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53726 USA;[10]Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China;[11]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA

年份：2023

卷号：314

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20231814035484);Scopus(收录号：2-s2.0-85153847575);WOS:【SCI-EXPANDED(收录号:WOS:000991346400001)】；

基金：This work was supported by the Fundamental Research Funds of the Chinese Academy of Forestry (CAFYBB2022XC001 to Q.L.) and the National Key Research and Development Program (2016YFD0600103 to Q.L.). F.L. and J.R. were funded by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science, DE-SC0018409). The XRD and SFG works carried by J.L., M.M., and S.H.K were supported the Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences (award #. DESC0001090). P.X. was sponsored by the Foundation of Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, College of Light Industry and Food Engineering, Guangxi University (2021KF36).

语种：英文

外文关键词：Nanocellulose microfibril; Poplar; Gibberellin; Pulp

摘要：Cellulose, the major component of secondary cell walls, is the most abundant renewable long-chain polymer on earth. Nanocellulose has become a prominent nano-reinforcement agent for polymer matrices in various in-dustries. We report the generation of transgenic hybrid poplar overexpressing the Arabidopsis gibberellin 20-ox-idase1 gene driven by a xylem-specific promoter to increase gibberellin (GA) biosynthesis in wood. X-ray diffraction (XRD) and sum frequency generation spectroscopic (SFG) analyses showed that cellulose in transgenic trees was less crystalline, but the crystal size was larger. The nanocellulose fibrils prepared from transgenic wood had an increased size compared to those from wild type. When such fibrils were used as a reinforcing agent in sheet paper preparation, the mechanical strength of the paper was significantly enhanced. Engineering the GA pathway can therefore affect nanocellulose properties, providing a new strategy for expanding nanocellulose applications.