文献类型：期刊文献

英文题名：Diverse Roles for a Class Ii Bpc Gene Through Tissue-Specific Regulation of Gene Expression in Plant Development

作者：Yu, Yifan[1,2,3] Ma, Xianjin[1,3] Huang, Mingchuan[1] Hu, Zhikang[1,3] Li, Sijia[1,2,3] Yin, Hengfu[1,3]

第一作者：Yu, Yifan

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang, Hangzhou, 311400, China; [2] College of Information Science and Technology, Nanjing Forestry University, Jiangsu, Nanjing, 210037, China; [3] Key Laboratory of Forest Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang, Hangzhou, 311400, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240106694)

语种：英文

外文关键词：Alkylation - Binding sites - Nucleotides - Plants (botany) - Polymerase chain reaction - Tissue - Transcription

摘要：BASIC PENTACYSTEINE (BPC) proteins are plant-specific transcription factors that bind to the GAGA dinucleotide repeats in the promoters of their target genes. They are classified into 4 classes based on sequence similarity but exhibit functional redundancy and specificity. BPC proteins regulate target gene expression by interacting with multiple transcription factors and chromatin-modifying proteins. However, the mechanisms by which BPCs regulate tissue-specific gene expression is not clear. In this study, we investigated the tissue-specific functions and mechanisms of CjBPC5, a class II BPC gene from Camellia japonica. The ectopic overexpression of CjBPC5 induced pleiotropic morphological defects in the leaves and floral tissues. Global gene expression profiling and DNA binding-site analyses showed that CjBPC5 regulated tissue-specific expression of several downstream genes by binding to the GAGA-rich binding sites in their promoters. Furthermore, we identified DNA motifs in the BPC binding sites that were critical for regulating tissue-specific gene expression and various developmental processes via transcription factor binding. Chromatin immunoprecipitation (ChIP) assay demonstrated direct binding of CjBPC5 to the promoter regions of EARLY FLOWERING 3 (ELF3), BEL1-LIKE HOMEODOMAIN 6 (BLH6), and BRASSINOSTEROID-6-OXIDASE 2 (BR6OX2). Quantitative real-time PCR (qRT-PCR) assays confirmed higher expression of the CjBPC5-target genes, including ELF3, BLH6, and BR6OX2 in the CjBPC5-overexpressing transgenic plants compared with the wild-type plants. In conclusion, our study demonstrated that CjBPC5 played a critical role in the plant developmental processes through tissue-specific regulation of the downstream genes by binding to specific cis-elements and interacting with specific transcription factors. ? 2024, The Authors. All rights reserved.