文献类型：期刊文献

英文题名：Transcriptome Profiling and Chlorophyll Metabolic Pathway Analysis Reveal the Response of Nitraria tangutorum to Increased Nitrogen

作者：Liu, Chenggong[1,2] Duan, Na[3,4] Chen, Xiaona[3,4] Li, Xu[1,2] Zhao, Naqi[3,4] Cao, Wenxu[1,2] Li, Huiqing[1,2] Liu, Bo[1,2] Tan, Fengsen[1,2] Zhao, Xiulian[1,2] Li, Qinghe[1,2]

第一作者：Liu, Chenggong

通信作者：Zhao, XL[1];Li, QH[1];Zhao, XL[2];Li, QH[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou 015200, Peoples R China;[4]Natl Forestry & Grassland Adm, Natl Long Term Sci Res Base Ulan Buh Desert Compre, Dengkou 015200, Peoples R China

年份：2023

卷号：12

期号：4

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000940574300001)】；

基金：This research was supported by the National Natural Science Foundation of China (Grant No. 31470622), the National Key R&D Program of China during the 13th Five-year Plan Period (Grant No. 2019YFF030320102), and the National Forest Germplasm Resource Platform Construction and Operation Services (Grant No. 2005DKA21003).

语种：英文

外文关键词：transcriptome analysis; nitrogen addition; Nitraria tangutorum; chlorophyll metabolism

摘要：To identify genes that respond to increased nitrogen and assess the involvement of the chlorophyll metabolic pathway and associated regulatory mechanisms in these responses, Nitraria tangutorum seedlings were subjected to four nitrogen concentrations (N0, N6, N36, and N60: 0, 6, 36, and 60 mmol center dot L-1 nitrogen, respectively). The N. tangutorum seedling leaf transcriptome was analyzed by high-throughput sequencing (Illumina HiSeq 4000), and 332,420 transcripts and 276,423 unigenes were identified. The numbers of differentially expressed genes (DEGs) were 4052 in N0 vs. N6, 6181 in N0 vs. N36, and 3937 in N0 vs. N60. Comparing N0 and N6, N0 and N36, and N0 and N60, we found 1101, 2222, and 1234 annotated DEGs in 113, 121, and 114 metabolic pathways, respectively, classified in the Kyoto Encyclopedia of Genes and Genomes database. Metabolic pathways with considerable accumulation were involved mainly in anthocyanin biosynthesis, carotenoid biosynthesis, porphyrin and chlorophyll metabolism, flavonoid biosynthesis, and amino acid metabolism. N36 increased delta-amino levulinic acid synthesis and upregulated expression of the magnesium chelatase H subunit, which promoted chlorophyll a synthesis. Hence, N36 stimulated chlorophyll synthesis rather than heme synthesis. These findings enrich our understanding of the N. tangutorum transcriptome and help us to research desert xerophytes' responses to increased nitrogen in the future.