文献类型：期刊文献

英文题名：Using lipidomics to reveal details of lipid accumulation in developing Siberian apricot (Prunus sibirica L.) seed kernels

作者：Mai, Yiting[1] Huo, Kaisen[1] Yu, Haiyan[2] Zhou, Nan[1] Shui, Lanya[1] Liu, Yang[1] Zhang, Chengxin[1] Niu, Jun[1,3] Wang, Libing[2]

第一作者：Mai, Yiting

通信作者：Niu, J[1];Wang, LB[2]

机构：[1]Hainan Univ, Sch Life & Pharmaceut, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Hainan Univ, Key Lab Genet & Germplasm Innovat Trop Special Fo, Haikou, Hainan, Peoples R China

年份：2020

卷号：12

期号：7

起止页码：539-552

外文期刊名：GLOBAL CHANGE BIOLOGY BIOENERGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000534631500001)】；

基金：The author acknowledges the financial support of the Central Public-Interest Scientific Institution Basal Research Fund (CAFYBB2017ZA004-4), the National Natural Science Foundation of China (31700586 and 41401069), and Hainan University research funded projects (KYQD(ZR)1701).

语种：英文

外文关键词：fatty acid; kernel development; lipidomics; phosphatidylcholine; polyunsaturated fatty acids; Prunus sibirica

摘要：Siberian apricot seed kernel (SASK) contains a high of 50% oil with suitable fuel properties conformed to biodiesel standard. To date, Prunus sibirica is a novel non-crop feedstock for biodiesel production in China. Here, oil contents and fatty acid (FA) compositions were identified in developing SASK from AS-80 and AS-84, at intervals of 1 week from 3 weeks after anthesis (WAA) to 9 weeks. The major differences in oil content between C18:1 and C18:2 levels were greater among the AS-80 (32.69/15.48 g/100 g) than among the AS-84 (25.78/13.15 g/100 g). Subsequently, the SASKs from 4, 6, and 8 WAA, respectively, representing early, middle, and late phases of oil accumulation, were selected as optimal samples for lipidomics analysis. It was notable that 18:1/18:1/18:2, 18:1/18:1/18:3, and 18:2/18:2/18:2 were the prominent compositions in triacylglycerol (TAG), and their higher content found among the AS-80 was consistent with FA results. Although phosphatidic acid (PA) is directly connected with diacylglycerol (DAG) in Kennedy pathway, we found significant difference between PA and DAG compositions. The resulting molecular species differ in acyl composition depending on whether they were generated via phosphatidylcholine (PC) or Kennedy pathway. By qRT-PCR analysis, the expression levels of FAD3, PDCT, and DAG-CPT related to the biosynthesis of polyunsaturated fatty acids (PUFAs) showed a gradual decrease with SASK mature, explaining the drastic change of DAG-18:3/18:3 content. Additionally, the lipidomics data coupled with qRT-PCR analysis suggested that phospholipid:DAG acyltransferase may play a critical role in incorporation of PUFAs into sn-3 of TAG. Our data contribute significantly to understand the underlying mechanisms of lipid accumulation in P. sibirica, and may also present strategies for engineering oil accumulation in oilseed plants.