文献类型：期刊文献

中文题名：黄牡丹花粉生活力测定方法的比较研究

英文题名：Comparison of Methods about Paeonia lutea's Pollen Viability Determination

作者：律春燕[1] 王雁[1] 朱向涛[1] 周琳[1] 郑宝强[1]

第一作者：律春燕

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

年份：2010

期号：2

起止页码：272-277

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：科技部863项目(2006AA100109,2007AA10Z182);国家林业局948项目(2006-4-C07)部分研究内容

语种：中文

中文关键词：黄牡丹;花粉;生活力;测定方法

外文关键词：Paeonia lutea ; pollen ; viability; determination method

分类号：S685.11

摘要：以黄牡丹的新鲜花粉为试材,利用单因子试验比较了液体培养基中蔗糖浓度、硼、钙、镁、钾对黄牡丹花粉萌发的影响,在此基础上进行了正交试验,比较了蔗糖、H3BO3及CaCl2对黄牡丹花粉萌发的影响;通过对醋酸洋红染色法、I-KI染色法和TTC染色法的比较,寻找快速测定黄牡丹花粉生活力的方法。试验结果表明:蔗糖及H3BO3对黄牡丹花粉萌发有极显著影响。在pH值为6.0时,蔗糖150 g.L-1+H3BO330 mg.L-1+CaCl220 mg.L-1适宜黄牡丹花粉培养,萌发率为68.7%;纯水培养没有造成花粉原生质体破裂,内含物外流,但萌发率极低,仅为3%;200 g.L-1以上的高浓度蔗糖溶液和300 mg.L-1以上的高浓度盐溶液会造成原生质体失水萎缩,质壁分离,这两种情况都抑制花粉萌发;TTC染色法测得的花粉活力率为64.9%,是快速测定黄牡丹花粉生活力的最适染色法。
The fresh pollen of Paeonia lutea was employed as experimental material to study the pollen viability. The effects of different concentration of sucrose, boron, calcium, magnesium and potassium on the germination of P. lutea＇ s pollen were compared through simple factorial experiment. Based on these, the orthogonal design was used to compare the effects of sucrose, boron and calcium on the germination of P. lutea＇ s pollen. Carmine acetate staining, I2-KI staining, and TTC staining were compared in order to seek for a fast determination method of P. lutea＇ s pollen viability. The results showed that the sucrose and boron had great effects on the germination of P. lutea＇ s pollen. Under the optimum pH 6.0 values, the optimum culture solution was sucrose 150 g ·L^-1 ＋ H3BO3 30 mg ·L^-1 ＋ CaC12 20 mg·L^-1 , the germination rate was 68.7%. When cultured in pure water, the protoplasm of the pollen wouldn＇ t split, and the substance inside wouldn＇ t flow out, but its germination rate was very low. When cultured in the solution with high concentration of sucrose or salt, the protoplasm would dehydrate and separate from the cell wall. These two would inhibit the germination of P. lutea＇ s pollen. And TIC staining showed that the viability rate was 64.9% which was the optimum staining for the fast determination of P. lutea＇ s pollen viability.