文献类型：期刊文献

英文题名：Exceptionally high genetic variance of the doubled haploid (DH) population of poplar

作者：Liu, Caixia[1,2] Wang, Sui[2,3] Liu, Yi[2] Wang, Meng[2] Fan, Erqin[2,4] Liu, Chen[2] Zhang, Shikai[2] Yang, Chuanping[2] Wang, Junhui[4] Sederoff, Heike W. W.[5] You, Xiangling[1] Chiang, Vincent L. L.[2,6] Chen, Su[2] Sederoff, Ronald R. R.[2,6] Qu, Guanzheng[2]

第一作者：Liu, Caixia

通信作者：Sederoff, RR[1];Qu, GZ[1];Sederoff, RR[2]

机构：[1]Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[3]Northeast Agr Univ, Chinese Minist Educ, Key Lab Soybean Biol, Harbin 150030, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry Ad, Beijing 100091, Peoples R China;[5]North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA;[6]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA

年份：2023

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;EI(收录号：20233514654809);Scopus(收录号：2-s2.0-85158059587);WOS:【SCI-EXPANDED(收录号:WOS:000982084100002)】；

语种：英文

外文关键词：Populus; Anther culture; Doubled haploid; Genetic variance; Flow cytometry

摘要：Doubled haploid (DH) plants have been widely used for breeding and biological research in crops. Populus spp. have been used as model woody plant species for biological research. However, the induction of DH poplar plants is onerous, and limited biological or breeding work has been carried out on DH individuals or populations. In this study, we provide an effective protocol for poplar haploid induction based on an anther culture method. A total of 96 whole DH plant lines were obtained using an F-1 hybrid of Populus simonii x P. nigra as a donor tree. The phenotypes of the DH population showed exceptionally high variance when compared to those of half-sib progeny of the donor tree. Each DH line displayed distinct features compared to those of the other DH lines or the donor tree. Additionally, some excellent homozygous lines have the potential to be model plants in genetic and breeding studies.