文献类型：期刊文献

英文题名：Exploring Transcriptional Regulation of Hyperaccumulation in Sedum plumbizincicola through Integrated Transcriptome Analysis and CRISPR/Cas9 Technology

作者：Zhang, Yixin[1,2] Mo, Yanlan[3] Han, Liyuan[1] Sun, Zhenyuan[1] Xu, Wenzhong[2,4,5]

第一作者：Zhang, Yixin

通信作者：Sun, ZY[1];Xu, WZ[2];Xu, WZ[3];Xu, WZ[4]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China;[3]Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China;[4]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[5]China Natl Bot Garden, Beijing 100093, Peoples R China

年份：2023

卷号：24

期号：14

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001036011700001)】；

语种：英文

外文关键词：cadmium; CRISPR; Cas9; hyperaccumulation; transcription factor; Sedum plumbizincicola; transcriptome sequencing

摘要：The cadmium hyperaccumulator Sedum plumbizincicola has remarkable abilities for cadmium (Cd) transport, accumulation and detoxification, but the transcriptional regulation mechanisms responsible for its Cd hyperaccumulation remain unknown. To address this knowledge gap, we conducted a comparative transcriptome study between S. plumbizincicola and the non-hyperaccumulating ecotype (NHE) of Sedum alfredii with or without Cd treatment. Our results revealed many differentially expressed genes involved in heavy metal transport and detoxification that were abundantly expressed in S. plumbizincicola. Additionally, we identified a large number of differentially expressed transcription factor genes, highlighting the complexity of transcriptional regulatory networks. We further screened four transcription factor genes that were highly expressed in the roots of S. plumbizincicola as candidate genes for creating CRISPR/Cas9 knockout mutations. Among these, the SpARR11 and SpMYB84 mutant lines exhibited decreased Cd accumulation in their aboveground parts, suggesting that these two transcription factors may play a role in the regulation of the Cd hyperaccumulation in S. plumbizincicola. Although further research will be required to determine the precise targeted genes of these transcription factors, combined transcriptome analysis and CRISPR/Cas9 technology provides unprecedented opportunities for identifying transcription factors related to Cd hyperaccumulation and contributes to the understanding of the transcriptional regulation mechanism of hyperaccumulation in S. plumbizincicola.