文献类型：期刊文献

中文题名：毛竹根际可培养微生物种群多样性分析

英文题名：Cultivable microbial diversity at the rhizosphere of Phyllostachys pubescens

作者：李潞滨[1] 刘敏[1] 杨淑贞[2] 刘亮[2] 缪崑[1] 杨凯[3] 韩继刚[4]

第一作者：李潞滨

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室;[2]浙江天目山国家级自然保护区管理局;[3]北京农学院农业应用新技术北京市重点实验室;[4]河北大学生命科学学院

年份：2008

卷号：48

期号：6

起止页码：772-779

中文期刊名：微生物学报

外文期刊名：Acta Microbiologica Sinica

收录：MEDLINE(收录号：18720842);CSTPCD;;Scopus(收录号：2-s2.0-66049150989);北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；PubMed;

基金：林业科技支撑计划(2006BAD01A1804)~~

语种：中文

中文关键词：毛竹;根际;可培养微生物;种群多样性

外文关键词：Phyllostachys pubescens; rhizosphere; culturable microbe; population diversity

分类号：Q938

摘要：【目的】为了了解天然毛竹林根际可培养微生物种群的多样性信息,【方法】采用稀释平板法,对浙江天目山和重庆缙云山天然毛竹林根际细菌和放线菌进行了分离,并对其16SrDNA序列进行了分析。【结果】分别从天目山和缙云山天然毛竹林根际分离得到51株和31株菌落形态差异的细菌和放线菌。16SrDNA序列分析表明,天目山和缙云山毛竹根际细菌主要包括厚壁菌门(Firmicutes,分别为40%和58%)、放线菌门(Actinobacteria,分别为36.7%和10.52%)、变形菌门α亚群(Alphaproteobacteria,分别为10%和5.26%)和变形菌门γ亚群(Gammaproteobacteria,分别为10%和26.32%),其中芽孢杆菌属(Bacillus sp.)为共同的优势菌属(分别为34.38%和42.11%)。分离的菌株中,B188、B171和B152等6株与GenBank中已报道16SrRNA基因序列的相似性从90%到96%不等,可能代表着新属或种。【结论】这表明,天然毛竹林根际具有较为丰富的可培养微生物种群多样性,并存在一些潜在的新的微生物菌种资源。
[Objective] To obtain the information of the cultivable microbial population diversity at the rhizosphere of Phyllostachys pubescens. [Methods] We isolated strains from Tianmu Mountain and Jinyun Mountain by diluting plate counting method and analyzed the 16S rDNA sequence of the isolates. [Results] We obtained 51 and 31 strains with different morphological character of colonies from Tianmu Mountain and Jinyun Mountain separately. The 16S rDNA sequence analysis showed that they had similar microbial population diversity. There were 40% and 58% firmicutes, 36.7% and 10.52% actinobacteria, 10% and 5.26% alphaproteobacteria, 10% and 26.32% gammaproteobacteria at the rhizosphere of Phyllostachys pubescens from Tianmu Mountain and Jinyun Mountain separately. The dominant bacteria were the genera Bacillus in both two areas. [Conclusion] The result showed that the cultivable microbial population diversity was abundant and there were some potential novel strains at the rhizosphere of Phyllostachys pubescens. Our research made it is possible to further investigate the function of the rhizosphere microbes and there interaction with the bamboo plant.