文献类型：期刊文献

英文题名：Complete chloroplast genome sequences of two endangered Phoebe (Lauraceae) species

作者：Li, Yingang[1] Xu, Wuqin[2] Zou, Wentao[3] Jiang, Dongyue[1] Liu, Xinhong[1]

第一作者：Li, Yingang

通信作者：Liu, XH[1]

机构：[1]Zhejiang Acad Forestry, Hangzhou 310023, Zhejiang, Peoples R China;[2]Zhejiang Univ, Key Lab Conservat Biol Endangered Wildlife, Coll Life Sci, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2017

卷号：58

期号：1

外文期刊名：BOTANICAL STUDIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000411041200001)】；

基金：This work was supported by the Major Science and Technology Program (No. 2010C12009), Key Research & Development Program (No. 2017C02028) of Zhejiang Province, and National Key Research & Development Program (No. 2016YFD0600603) of China.

语种：英文

外文关键词：Phoebe chekiangensis; Phoebe bournei; Chloroplast genomes; Repeat analysis; SSRs; Divergent regions

摘要：Background: Phoebe (Lauraceae) comprises of evergreen trees or shrubs with approximately 100 species, distributed in tropical and subtropical Asia and Neotropical America. A total of 34 species and three varieties occur in China. Despite of economic and ecological value, only limited genomic resources are available for this genus. Results: We sequenced the two complete chloroplast (cp) genomes of Phoebe chekiangensis and P. bournei using Illumina sequencing technology via a combined strategy of de novo and reference-guided assembly. We also performed comparative analyses with the cp genomes of P. sheareri and P. sheareri var. oineiensis previously reported. The chloroplast genomes of P. chekiangensis and P. bournei identically contain 112 genes consisting of 78 protein coding genes, 30 tRNA genes, and 4 rRNA genes, with the size of 152,849 and 152,853 bp, respectively. From the two chloroplast genomes, 131 SSRs were identified and 12 different SSRs located in five protein coding genes. The analysis showed the extremely conserved structure of chloroplast genomes with surprisingly little variations at the LSC/IR and SSC/IR boundaries. Moreover, the mean nucleotide diversity was found to be 0.162% for 77 regions, suggesting an extraordinarily low level of sequence divergence. Four highest divergent regions (trnH-psbA, rps14-trnT, petA-psbJ, ccsA-ndhD) with the percentage of nucleotide diversity higher than 0.50% were identified, which had potential use for species identification and phylogenetic studies. Conclusion: This study will facilitate our understanding of population genetics, phylogenetic relationship and plant evolution of Phoebe species.