文献类型：期刊文献

英文题名：Analysis of genetic diversity and population structure in endangered Populus wulianensis based on 18 newly developed EST-SSR markers

作者：Wu, Qichao[1] Zang, Fengqi[2] Ma, Yan[1] Zheng, Yongqi[2] Zang, Dekui[1]

第一作者：Wu, Qichao

通信作者：Zang, DK[1]

机构：[1]Shandong Agr Univ, Coll Forestry, Key Lab State Forestry Adm Silviculture Lower Yel, Tai An 271018, Shandong, Peoples R China;[2]Chinese Acad Forestry, Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：24

外文期刊名：GLOBAL ECOLOGY AND CONSERVATION

收录：;Scopus(收录号：2-s2.0-85095457231);WOS:【SCI-EXPANDED(收录号:WOS:000608479300019)】；

基金：This study was supported by the Shandong Agricultural Seeds Engineering Project (2019LZGC01802) and the National Forest Germplasm Resources Platform Project 2019-2020.

语种：英文

外文关键词：Endangered plant; EST-SSR markers; Genetic diversity; Population structure; Populus wulianensis

摘要：Populus wulianensis is an endangered tree species endemic to Shandong Province, China. Genetic diversity assessments represent the basis and a prerequisite for protecting endangered species. In our study, a total of 116 individuals of P. wulianensis from 4 extant populations (3 wild populations and 1 artificial population that covered the entire geographic distribution of the species) were analyzed using 18 expressed sequence tag simple sequence repeat (EST-SSR) markers to assess their genetic diversity and population structure. The 18 EST-SSR markers had high levels of polymorphisms (PIC = 0.698), and 202 alleles were amplified. The present study revealed high genetic diversity at the population level (Na = 4.847, NR = 3.000, HS = 0.624, HE = 0.611, I =1.114, and PPL = 98.6%) and the species level (HT = 0.741). Private alleles were detected in all four populations (including the artificial population JYS). Negative inbreeding coefficients (FIS<0) indicated that the populations of P. wulianensis produced too many heterozygotes due to outbreeding and that there might be a risk of outbreeding depression. The AMOVA results showed that 19.05% and 80.95% of the total variation occurred among and within populations, respectively. Obvious genetic differentiation between populations (FST = 0.191) and a high level of gene flow (Nm = 2.566) were detected, indicating that gene flow is not the main cause of genetic differentiation among populations. The results of UPGMA cluster analysis, PCoA and structure analysis (DK = 4) showed that the 4 populations were clustered into four groups and suggested that the natural population ZHS gave rise to the artificial population JYS. The genetic bottleneck analysis results suggested that three populations (ZHS, JXS and JYS) recently experienced bottleneck events. These genetic results are of great significance for scientifically formulating conservation strategies for endangered P. wulianensis. (C) 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.