文献类型：期刊文献

英文题名：Genetic diversity in the endangered Camellia nitidissima assessed using transcriptome-based SSR markers

作者：Li, Xin-lei[1,2] Wang, Jie[1,2] Fan, Zheng-qi[1,2] Li, Ji-yuan[1,2] Yin, Heng-fu[1,2]

第一作者：李辛雷

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Key Lab Forest Genet & Breeding, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2020

卷号：34

期号：2

起止页码：543-552

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000527948200018)】；

基金：We thank three anonymous reviewers for critical comments that helped to improve the manuscript. This work is supported by Special Fund for Forest Scientific Research in the Public Welfare (201504707); National Science Foundation of China (31470697, 31870578); Nonprofit Research Projects of Chinese Academy of Forestry (CAFYBB2017SZ001); International Sci. & Tech. Cooperation Program of China (2016YFE0126100).

语种：英文

外文关键词：Camellia nitidissima; Genetic diversity; Genetic structure; SSR; Germplasm conservation

摘要：Camellia nitidissima is an endangered species whose flowers are bright yellow. In the past 50 years, habitat changes caused by climate and human activity have greatly reduced the extent and size of natural populations of C. nitidissima. Here, to provide a fundamental basis for this plant's protection and utilization, the genetic diversity and genetic structure of C. nitidissima natural populations were studied using simple sequence repeats (SSR) markers. Based on a comparative analysis of multiple transcriptomes from different Camellia species, a group of consensus transcript sequences were obtained for marker development. We screened 237 predicted transcript loci, and identified 21 highly polymorphic SSR markers for population analysis. In total, 82 alleles were obtained to assess the genetic diversity of four natural populations in Guangxi Zhuang autonomous region, China. The results revealed that the mean observed heterozygosity (Ho) and expected heterozygosity (He) of the populations were 0.624 and 0.546, respectively, and the genetic diversity was mostly among populations. Further, we uncovered a significant positive correlation between the genetic distance and geographic distance of C. nitidissima populations. The high genetic diversity found in this study holds promise for the future conservation and restoration of C. nitidissima populations based on efforts from in situ and ex situ strategies implemented in a carefully monitored way.