文献类型：期刊文献

英文题名：Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress

作者：Wang, Dongli[1] Meng, Sen[1,2] Su, Wanlong[1] Bao, Yu[1] Lu, Yingying[1] Yin, Weilun[1] Liu, Chao[1] Xia, Xinli[1]

第一作者：Wang, Dongli

通信作者：Liu, C[1];Xia, XL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Guangzhou 510520, Guangdong, Peoples R China

年份：2019

卷号：20

期号：6

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85063673184);WOS:【SCI-EXPANDED(收录号:WOS:000465523400065)】；

基金：This research was sponsored by grants from the National Key Program on Transgenic Research (2018ZX08021001) and the National Natural Science Foundation of China (31770649, 31570308, and 31600484).

语种：英文

外文关键词：Populus trichocarpa; multiple organellar RNA editing factor; drought stress; RNA editing; genome

摘要：Poplar (Populus) is one of the most important woody plants worldwide. Drought, a primary abiotic stress, seriously affects poplar growth and development. Multiple organellar RNA editing factor (MORF) genespivotal factors in the RNA editosome in Arabidopsis thalianaare indispensable for the regulation of various physiological processes, including organelle C-to-U RNA editing and plasmid development, as well as in the response to stresses. Although the poplar genome sequence has been released, little is known about MORF genes in poplar, especially those involved in the response to drought stress at the genome-wide level. In this study, we identified nine MORF genes in the Populus genome. Based on the structural features of MORF proteins and the topology of the phylogenetic tree, the P. trichocarpa (Ptr) MORF family members were classified into six groups (Groups I-VI). A microsynteny analysis indicated that two (22.2%) PtrMORF genes were tandemly duplicated and seven genes (77.8%) were segmentally duplicated. Based on the d(N)/d(S) ratios, purifying selection likely played a major role in the evolution of this family and contributed to functional divergence among PtrMORF genes. Moreover, analysis of qRT-PCR data revealed that PtrMORFs exhibited tissue- and treatment-specific expression patterns. PtrMORF genes in all group were involved in the stress response. These results provide a solid foundation for further analyses of the functions and molecular evolution of MORF genes in poplar, and, in particular, for improving the drought resistance of poplar by genetics manipulation.