文献类型：期刊文献

中文题名：日本落叶松体细胞胚胎发生相关基因LaSERK1的克隆与表达分析

英文题名：Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1(SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

作者：李龙[1] 张立峰[2] 齐力旺[2] 韩素英[1]

第一作者：李龙

机构：[1]中国林业科学研究院森林生态环境与保护研究所;[2]中国林业科学研究院林业研究所细胞生物学实验室

年份：2013

卷号：26

期号：6

起止页码：673-680

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家重点基础研究发展计划(973)项目"速生优质林木培育的遗传基础及分子调控"(2009CB119100);国家高技术研究发展计划(863)项目"高产优质多抗林木花草分子与细胞高效育种技术及品种创制"(2006AA100109)

语种：中文

中文关键词：日本落叶松;体细胞胚胎发生;SERK1;表达分析

外文关键词：Larix kaempferi; somatic embryogenesis; SERK1; expression pattern

分类号：S718.46

摘要：本研究克隆了日本落叶松体细胞胚胎发生相关受体类蛋白激酶基因LaSERK1,并检测了在不同培养条件下LaSERK1的表达情况,发现LaSERK1与已发现的其他物种SERK1基因有高度相似性,qRT-PCR结果显示:LaSERK1在体胚发生早期高表达。结果表明:LaSERK1可能在落叶松体胚发育早期起重要作用,LaSERK1也可能成为早期胚性细胞的标记基因。
Somatic embryogenesis not only plays a significant role in understanding the mechanism of embryogene- sis, but also in improving the breeding of Larix kaempferi （Lamb.） Carr. The somatic embryogenesis receptor-like kinase 1 （SERK1） gene is considered as an essential element involved in the early stage of this process. The ex- pression pattern of the homolog of SERK! of L. kaempferi in different culture conditions was studied. LaSERK1 shared all the characteristic domains of the SERK family. The qRT-PCR showed that LaSERK1 was highly expressed in L. kaempferi early embryogenic cells cultured in the mature medium. These results suggest that LaSERK1 might perform a significant role during early somatic embryogenesis of L. kaempferi, and might be a potential marker of ear- ly embryogenic cells.