文献类型：期刊文献

英文题名：Comparative Analysis of the Fecal Bacterial Microbiota of Wintering Whooper Swans (Cygnus Cygnus)

作者：Wang, Wenxia[1] Huang, Songlin[2,3] Yang, Liangliang[2,3] Zhang, Guogang[2,3]

第一作者：王文霞

通信作者：Yang, LL[1];Yang, LL[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry Policy & Informat, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing, Peoples R China;[3]Key Lab Forest Protect Natl Forestry & Grassland, Beijing, Peoples R China

年份：2021

卷号：8

外文期刊名：FRONTIERS IN VETERINARY SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000678743300001)】；

基金：This work was funded by the National Natural Science Foundation of China (No. 31600323), Basic Research Business Special Project of Chinese Academy of Forestry (No. CAFYBB2016SY019), and Forewarning of Avian Influenza along theWestern Flyway in China (No. 91203-2021).

语种：英文

外文关键词：wintering whooper swans; bacterial microbiota; conservation; LEfSe analysis; 16S-rRNA gene sequencing

摘要：There are many and diverse intestinal microbiota, and they are closely related to various physiological functions of the body. They directly participate in the host's food digestion, nutrient absorption, energy metabolism, immune response, and many other physiological activities and are also related to the occurrence of many diseases. The intestinal microbiota are extremely important for maintaining normal physical health. In order to explore the composition and differences of the intestinal microbiota of whooper swans in different wintering areas, we collected fecal samples of whooper swans in Sanmenxia, Henan, and Rongcheng, Shandong, and we used the Illumina HiSeq platform to perform high-throughput sequencing of bacterial 16S rRNA genes. Comparison between Sanmenxia and Rongcheng showed no significant differences in ACE, Chao 1, Simpson, and Shannon indices (p > 0.05). Beta diversity results showed significant differences in bacterial communities between two groups [analysis of similarity (ANOSIM): R = 0.80, p = 0.011]. Linear discriminant analysis effect size (LEfSe) analysis showed that at the phylum level, the relative abundance of Actinobacteria was significantly higher in Sanmenxia whooper swans than Rongcheng whooper swans. At the genus level, the amount of Psychrobacter and Carnobacterium in Sanmenxia was significantly higher in Rongcheng, while the relative abundance Catellicoccus and Lactobacillus was significantly higher in Rongcheng than in Sanmenxia. This study analyzed the composition, characteristics, and differences of the intestinal microbiota of the whooper swans in different wintering environments and provided theoretical support for further exploring the relationship between the intestinal microbiota of the whooper swans and the external environment. And it played an important role in the overwintering physiology and ecology, population management, and epidemic prevention and control of whooper swans.