文献类型：期刊文献

中文题名：大花惠兰根腐病拮抗细菌ZL7-5菌株的筛选与鉴定

英文题名：Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium

作者：李潞滨[1] 李术娜[2] 李佳[2] 王倩[2] 朱宝成[2] 彭镇华[1]

第一作者：李潞滨

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]河北农业大学生命科学学院,河北保定071001

年份：2008

卷号：35

期号：11

起止页码：1647-1652

中文期刊名：园艺学报

外文期刊名：Acta Horticulturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：引进国际先进林业科学技术创新项目(2006-4-C08);国家‘十一五’科技支撑计划项目(2006BAD01A18)

语种：中文

中文关键词：大花惠兰;根腐病;尖孢镰刀菌;拮抗细菌;筛选;鉴定;解淀粉芽孢杆菌

外文关键词：Cymbidium hybridium ; root rot; Fusarium oxysporium ; antagonistic bacteria; screening; identification ; Bacillus amyloliquefaciens

分类号：S682.31

摘要：从各地大花惠兰种植地采集的土样中共分离到细菌519株,初筛出对大花惠兰根腐病病原菌尖孢镰刀菌(Fusarium oxysporium)具有拮抗活性的细菌菌株26株,经过复筛,选出1株具有较高拮抗活性的菌株ZL7-5。通过16S rDNA序列相似性分析,菌株ZL7-5与解淀粉芽孢杆菌标准菌株(NBRC15535)的16S rDNA序列相似度达100%,通过比较菌株ZL7-5和模式菌株的形态特征和生理生化特性,最终将菌株ZL7-5鉴定为解淀粉芽孢杆菌(Bacillus amylolique faciens)。
In order to obtain antagonistic strain which has the potential application possibility on biocontrol of root rot disease of Cymbidium hybridiurn, five hundreds and nineteen bacterial strains were isolated from the soil samples and there were twenty-six bacterial strains showed antagonistic activity to Fusariurn oxysporium. A strain with the highest antagonistic activity, ZL7-5, was obtained during the secondary screening. 16S rDNA sequences analysis of strain ZL7-5 revealed that it had 100% similarity to that of Bacillus arnyloliquefaciens NBRC 15535. After the morphological, physiological and biochemical characteristics of this strain were further studied and compared with that of the type strain Bacillus amyloliquefaciens NBRC 15535, strain ZL7-5 was finally identified as Bacillus amyloliquefaciens.