文献类型：期刊文献

英文题名：Genomic landscape of a relict fir-associated fungus reveals rapid convergent adaptation towards endophytism

作者：Yuan, Zhilin[1,2] Wu, Qi[3] Xu, Liangxiong[4] Druzhinina, Irina S.[5,6] Stukenbrock, Eva H.[7,8] Nieuwenhuis, Bart P. S.[9] Zhong, Zhenhui[10,11] Liu, Zhong-Jian[12] Wang, Xinyu[2] Cai, Feng[5] Kubicek, Christian P.[6] Shan, Xiaoliang[1,2] Wang, Jieyu[13] Shi, Guohui[3] Peng, Long[1,2] Martin, Francis M.[14]

第一作者：袁志林

通信作者：Yuan, ZL[1];Yuan, ZL[2];Martin, FM[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[3]Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China;[4]Huizhou Univ, Sch Life Sci, Huizhou 516007, Peoples R China;[5]Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Plant Immun, Fungal Genom Lab FungiG, Nanjing 210095, Peoples R China;[6]TU Wien, Inst Chem Environm & Biosci Engn ICEBE, A-1060 Vienna, Austria;[7]Christian Albrechts Univ Kiel, Bot Inst, D-24118 Kiel, Germany;[8]Max Planck Inst Evolutionary Biol, Environm Genom Res Grp, D-24306 Plon, Germany;[9]Ludwig Maximilians Univ Munchen, Div Evolutionary Biol, D-82152 Planegg Martinsried, Germany;[10]Fujian Agr & Forestry Univ, Coll Plant Protect, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China;[11]Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA;[12]Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Natl Forestry & Grassland Adm Orchid Cons, Fuzhou 350002, Peoples R China;[13]Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Peoples R China;[14]Univ Lorraine, INRAe, INRAe Grand Est Nancy, UMR Interact Arbres Microorganismes 1136, F-54280 Champenoux, France

年份：2022

卷号：16

期号：5

起止页码：1294-1305

外文期刊名：ISME JOURNAL

收录：;Scopus(收录号：2-s2.0-85121454486);WOS:【SCI-EXPANDED(收录号:WOS:000730887000001)】；

基金：Research in Yuan's lab is financially supported by the Fundamental Research Funds for the Central Non-profit Research of the Chinese Academy of Forestry (CAFYBB2019ZA001-3) and the National Natural Science Foundation of China (No. 31722014 and 31772032). Research in Martin's lab is supported by the Laboratory of Excellence ARBRE (ANR-11-LABX-0002-01) and the Beijing Advanced Innovation Center for Tree Breeding by Molecular Design. We extend our sincere gratitude to Dr. Primrose Boynton (Wheaton College, USA) and Dr. Fengyan Bai (State Key Laboratory of Mycology, Chinese Academy of Sciences) for useful discussions. We thank Dr Yougui Wu (Fengyangshan-Baishanzu National Nature Reserve, China) for providing basic information about the wild relict fir trees.

语种：英文

摘要：Comparative and pan-genomic analyses of the endophytic fungus Pezicula neosporulosa (Helotiales, Ascomycota) from needles of the relict fir, Abies beshanzuensis, showed expansions of carbohydrate metabolism and secondary metabolite biosynthetic genes characteristic for unrelated plant-beneficial helotialean, such as dark septate endophytes and ericoid mycorrhizal fungi. The current species within the relatively young Pliocene genus Pezicula are predominantly saprotrophic, while P. neosporulosa lacks such features. To understand the genomic background of this putatively convergent evolution, we performed population analyses of 77 P. neosporulosa isolates. This revealed a mosaic structure of a dozen non-recombining and highly genetically polymorphic subpopulations with a unique mating system structure. We found that one idiomorph of a probably duplicated mat1-2 gene was found in putatively heterothallic isolates, while the other co-occurred with mat1-1 locus suggesting homothallic reproduction for these strains. Moreover, 24 and 81 genes implicated in plant cell-wall degradation and secondary metabolite biosynthesis, respectively, showed signatures of the balancing selection. These findings highlight the evolutionary pattern of the two gene families for allowing the fungus a rapid adaptation towards endophytism and facilitating diverse symbiotic interactions.