文献类型：期刊文献

中文题名：云南红豆杉紫杉醇含量变异及其相关的RAPD分子标记

英文题名：RAPD Markers Related to Taxol Content of Taxus yunnanensis

作者：苏建荣[1] 缪迎春[1] 张志钧[1]

第一作者：苏建荣

机构：[1]中国林业科学研究院资源昆虫研究所

年份：2009

卷号：45

期号：7

起止页码：16-20

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家"十一五"科技支撑计划(2006BAD18B03);国家林业局林业科学技术研究项目(2008-06);中国林业科学研究院资源昆虫研究所计划项目(RIRI200703Z);云南省自然科学基金(2008CD200)

语种：中文

中文关键词：云南红豆杉;紫杉醇含量;变异;RAPD

外文关键词：Taxus yunnanensls ; taxol content; variation; RAPD

分类号：S718.43;S718.46

摘要：从5个云南红豆杉群体中分别选取树龄与生长状况基本一致的植株各10个,按统一的取样标准采集树皮、小枝和针叶样品测定紫杉醇含量,分析其变异分布式样;同时对所有采样植株进行RAPD分析,开展RAPD标记与不同器官紫杉醇含量的关联分析。结果表明:不同云南红豆杉群体间树皮、小枝和针叶的紫杉醇含量均差异显著(P<0.05),树皮、小枝和针叶紫杉醇含量在群体间的变异分别占其总变异的16.22%,33.90%和45.81%。通过RAPD标记与紫杉醇含量的关联分析,分别筛选出与云南红豆杉树皮、小枝和针叶紫杉醇含量相关联的位点11,13,59个,其贡献率依次为7.791%～11.509%,7.853%～24.457%,7.858%～26.729%;同时筛选出了可指示树皮、小枝和针叶低紫杉醇含量的特异标记。云南红豆杉的遗传改良应根据不同器官紫杉醇含量变异的分布特点结合叶用、枝用和皮用等培育目标采取不用的选育策略。
Ten trees with approximately the same age and diameter at breast height were sampled from each of the 5 populations of Taxus yunnanensis to measure taxol contents of barks, twigs and needles. Their variation patterns were then analyzed, and correlations between 194 RAPD markers and taxol contents of barks, twigs and needles were further tested. The result indicated that there were significant （P 〈 0.05 ） differences of taxol content in bark, twigs and needles among 5 populations. The variations in barks, twigs and needles took up 16.22%, 33.9% and 45.81%, respectively among populations. There were 11, 13 and 59 RAPD markers identified to be significantly correlated to taxol contents of barks, twigs and needles with their contribution rates ranging from 7.791% to 11.509%, 7.853% to 24.457% and 7.858% to 26.729%, respectively. Special markers were also screened out to indicate the low taxol content in barks, twigs and needles. It was suggested that different genetic improvement strategies should be used for various management objectives such as barks, twigs or needles utility, in terms of the variations of taxol contents among different organs in this species.