详细信息
文献类型:期刊文献
中文题名:成熟龄杂交鹅掌楸再生体系的建立
英文题名:Establishment of plant regeneration system in matured Liriodendron hybrids
作者:李纪元[1] 田敏[1] 李辛雷[1] 范正琪[1] 倪穗[2]
第一作者:李纪元
机构:[1]中国林业科学研究院亚热带林业研究所;[2]宁波大学生命科学与生物工程学院
年份:2006
卷号:23
期号:5
起止页码:512-515
中文期刊名:浙江林学院学报
外文期刊名:Journal of Zhejiang Forestry College
收录:CSTPCD;;北大核心:【北大核心2004】;CSCD:【CSCD_E2011_2012】;
基金:浙江省科学技术重点项目(2003C22011;2006C22061)
语种:中文
中文关键词:林木育种学;鹅掌楸属;杂交种;分化;再生体系
外文关键词:forest tree breeding; Liriodendron; hybrids; differentiation; plant regeneration
分类号:S722.3
摘要:以叶片及叶柄为外植体,建立了杂交鹅掌楸Liriodendron chinense×L.tulipifera成年树的离体培养再生体系。结果表明,外植体在多种植物生长调节物质组合的培养基上均能产生愈合组织。在MS+0.5mg·L^-1NAA+2.0~4.0mg·L^-16-BA的培养基上,愈合组织能分化产生不定芽。在MS+0.5mg·L^-1NAA+4.0mg·L^-16-BA的培养基上,外植体可不经过愈合组织而直接分化产生不定芽。最适生根培养基为1/2MS+1.5mg·L^-1NAA。
The in vitro plant regeneration system of Liriodendron hybrids planted in 1967 at the yard of Research Institute of Subtropical Forestry was established by using the leaves and petioles as explants. The results showed that callus could be induced from these explants on the media supplemented with different concentrations of plant growth regulators. The adventitious buds could be generated from the calli induced on the media MS + 0.5 mg·L^-1 NAA + 2.0 - 4.0 mg·L^-1 6-BA. On the medium MS + 0.5 mg·L^-1 NAA + 4.0 mg·L^-1 6-BA, the adventitious buds could be directly differentiated from the explants without inducing calli. The best medium for rooting was 1/2 MS + 1.5 mg·L^-1 NAA.
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