文献类型：期刊文献

英文题名：Altered microbial structure and function after thermokarst formation

作者：Liu, Futing[1,2,3] Kou, Dan[1,2] Chen, Yongliang[1] Xue, Kai[2] Ernakovich, Jessica G.[4] Chen, Leiyi[1] Yang, Guibiao[1,2] Yang, Yuanhe[1,2]

第一作者：Liu, Futing;刘富庭

通信作者：Yang, YH[1]

机构：[1]Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China;[2]Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[4]Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA

年份：2021

卷号：27

期号：4

起止页码：823-835

外文期刊名：GLOBAL CHANGE BIOLOGY

收录：;Scopus(收录号：2-s2.0-85097017958);WOS:【SCI-EXPANDED(收录号:WOS:000592386400001)】；

基金：National Natural Science Foundation of China, Grant/Award Number: 31825006, 31988102 and 91837312; Second Tibetan Plateau Scientific Expedition and Research, Grant/Award Number: 2019QZKK0106 and 2019QZKK0302; Chinese Academy of Sciences, Grant/Award Number: QYZDBSSW-SMC049

语种：英文

外文关键词：carbon cycle; carbon– climate feedback; functional gene; metagenomic sequencing; microbe; permafrost thaw

摘要：Permafrost thaw could induce substantial carbon (C) emissions to the atmosphere, and thus trigger a positive feedback to climate warming. As the engine of biogeochemical cycling, soil microorganisms exert a critical role in mediating the direction and strength of permafrost C-climate feedback. However, our understanding about the impacts of thermokarst (abrupt permafrost thaw) on microbial structure and function remains limited. Here we employed metagenomic sequencing to analyze changes in topsoil (0-15 cm) microbial communities and functional genes along a permafrost thaw sequence (1, 10, and 16 years since permafrost collapse) on the Tibetan Plateau. By combining laboratory incubation and a two-pool model, we then explored changes in soil labile and stable C decomposition along the thaw sequence. Our results showed that topsoil microbial alpha-diversity decreased, while the community structure and functional gene abundance did not exhibit any significant change at the early stage of collapse (1 year since collapse) relative to non-collapsed control. However, as the time since the collapse increased, both the topsoil microbial community structure and functional genes differed from the control. Abundances of functional genes involved in labile C degradation decreased while those for stable C degradation increased at the late stage of collapse (16 years since collapse), largely driven by changes in substrate properties along the thaw sequence. Accordingly, faster stable C decomposition occurred at the late stage of collapse compared to the control, which was associated with the increase in relative abundance of functional genes for stable C degradation. These results suggest that upland thermokarst alters microbial structure and function, particularly enhances soil stable C decomposition by modulating microbial functional genes, which could reinforce a warmer climate over the decadal timescale.