文献类型：期刊文献

英文题名：Functional differentiation of olive PLP_deC genes: insights into metabolite biosynthesis and genetic improvement at the whole-genome level

作者：Cui, Qizhen[1,2,3] Liu, Qingqing[1,2,3] Fan, Yutong[1,2,3] Wang, Chenhe[1,2,3] Li, Yufei[1,2,3] Li, Shuyuan[1,2,3] Zhang, Jianguo[1,2,3] Rao, Guodong[1,2,3]

第一作者：Cui, Qizhen

通信作者：Rao, GD[1];Rao, GD[2];Rao, GD[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2024

卷号：43

期号：5

外文期刊名：PLANT CELL REPORTS

收录：;Scopus(收录号：2-s2.0-85191093798);WOS:【SCI-EXPANDED(收录号:WOS:001220872500005)】；

基金：No Statement Available

语种：英文

外文关键词：Pyridoxal phosphate-dependent decarboxylases; Hydroxytyrosol; Oleuropein; Olive

摘要：Key MessageThis study identified 16 pyridoxal phosphate-dependent decarboxylases in olive at the whole-genome level, conducted analyses on their physicochemical properties, evolutionary relationships and characterized their activity.AbstractGroup II pyridoxal phosphate-dependent decarboxylases (PLP_deC II) mediate the biosynthesis of characteristic olive metabolites, such as oleuropein and hydroxytyrosol. However, there have been no report on the functional differentiation of this gene family at the whole-genome level. This study conducted an exploration of the family members of PLP_deC II at the whole-genome level, identified 16 PLP_deC II genes, and analyzed their gene structure, physicochemical properties, cis-acting elements, phylogenetic evolution, and gene expression patterns. Prokaryotic expression and enzyme activity assays revealed that OeAAD2 and OeAAD4 could catalyze the decarboxylation reaction of tyrosine and dopa, resulting in the formation of their respective amine compounds, but it did not catalyze phenylalanine and tryptophan. Which is an important step in the synthetic pathway of hydroxytyrosol and oleuropein. This finding established the foundational data at the molecular level for studying the functional aspects of the olive PLP_deC II gene family and provided essential gene information for genetic improvement of olive.