文献类型：期刊文献

英文题名：The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

作者：Shu, Wenbo[1,2] Zhou, Houjun[1] Jiang, Cheng[1] Zhao, Shutang[1] Wang, Liuqiang[1] Li, Quanzi[1] Yang, Zhangqi[2] Groover, Andrew[3] Lu, Meng-Zhu[1]

第一作者：Shu, Wenbo

通信作者：Lu, MZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Guangxi Acad Forestry, Nanning, Guangxi, Peoples R China;[3]US Forest Serv, Pacific Southwest Res Stn, Davis, CA USA

年份：2019

卷号：17

期号：2

起止页码：338-349

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000456283800003)】；

基金：This work was supported by the Special Fund for Forest Scientific Research in the Public Welfare (Grant no. 201504101), Chinese Academy of Forestry, China. We thank Professor Mark Estelle (Howard Hughes Medical Institute and Section of Cell and Developmental Biology, University of California San Diego, USA) for help to the LexA yeast two-hybrid experiments. We also thank Dr Fang Tang and Dr Chao Sun of State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry for their valuable technical contribution. The authors declare no conflict of interest.

语种：英文

外文关键词：adventitious root development; auxin signalling; PagFBL1; PagIAA28

摘要：Adventitious roots occur naturally in many species and can also be induced from explants of some tree species including Populus, providing an important means of clonal propagation. Auxin has been identified as playing a crucial role in adventitious root formation, but the associated molecular regulatory mechanisms need to be elucidated. In this study, we examined the role of PagFBL1, the hybrid poplar (Populus alba x P. glandulosa clone 84K) homolog of Arabidopsis auxin receptor TIR1, in adventitious root formation in poplar. Similar to the distribution pattern of auxin during initiation of adventitious roots, PagFBL1 expression was concentrated in the cambium and secondary phloem in stems during adventitious root induction and initiation phases, but decreased in emerging adventitious root primordia. Overexpressing PagFBL1 stimulated adventitious root formation and increased root biomass, while knock-down of PagFBL1 transcript levels delayed adventitious root formation and decreased root biomass. Transcriptome analyses of PagFBL1 overexpressing lines indicated that an extensive remodelling of gene expression was stimulated by auxin signalling pathway during early adventitious root formation. In addition, PagIAA28 was identified as downstream targets of PagFBL1. We propose that the PagFBL1-PagIAA28 module promotes adventitious rooting and could be targeted to improve Populus propagation by cuttings.