文献类型：期刊文献

英文题名：Gene-Specific Effects of Three Cry Transgenes on Rhizosphere Microbiota in Catalpa bungei

作者：Mao, Xiaofeng[1] Lv, Fenni[1,2] Li, Shaofeng[3] Gao, Lulu[1,2] Ma, Wenjun[4] Liu, Donglai[1] Wu, Binpeng[1] Wu, Yanan[1] Wang, Peng[1,2] Li, Naiwei[1]

第一作者：Mao, Xiaofeng

通信作者：Wang, P[1];Li, NW[1];Wang, P[2]

机构：[1]Jiangsu Prov & Chinese Acad Sci, Inst Bot, Jiangsu Key Lab Conservat & Utilizat Plant Resourc, Nanjing, Peoples R China;[2]Jiangsu Engn Res Ctr Landscape Plant Resources & G, Nanjing, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, State Key Lab Tree Genet & Breeding, Natl Permanent Sci Res Base Warm Temperate Zone Fo, Beijing, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing, Peoples R China

年份：2026

外文期刊名：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001665445100001)】；

基金：Funding Statement: This research was funded by the Chinese Academy of Forestry-Special funds for basic scientific research service expenses of the central level public welfare research institutes (Grant No. CAFYBB2020QD001) , the National Natural Science Foundation of China (Grant Nos. 32101550, 32271917) , Jiangsu Agricultural Science and Technology Innovation Fund (Grant No. CX (24) 3052) , National Forestry and Grassland Administration's Center for Science and Technology Development Projects (Grant No. KJZXSA202202) .

语种：英文

外文关键词：Catalpa bungei; Cry genes; rhizosphere microbiome; 16S rDNA sequencing; environmental biosafety

摘要：Catalpa bungei, a fast-growing timber tree, is threatened by the lepidopteran pest Omphisa plagialis. Previous studies in our laboratory successfully generated transgenic C. bungei lines overexpressing Cry genes (Cry1Ab, Cry2A, and Cry9-2) that exhibited resistance to O. plagialis, but their potential impact on soil bacterial communities remains unclear. In this study, we analyzed nine transgenic C. bungei lines (three independent lines for each Cry gene) to characterize their rhizosphere bacterial communities using high-throughput sequencing of the 16S ribosomal DNA (rDNA) V4-V5 regions. A total of 628 amplicon sequence variants (ASVs) were shared among all transgenic and wild-type (WT) lines, forming a stable core microbiome dominated by Proteobacteria, Bacteroidota, Acidobacteriota, and Actinobacteriota. Alpha diversity showed no significant differences, while beta diversity revealed minor but distinct compositional shifts. Cry1Ab lines exhibited higher abundances of fast-growing taxa, particularly Proteobacteria and Bacteroidota; Cry2A lines displayed intermediate profiles, whereas Cry9-2 lines were nearly indistinguishable from WT communities. Linear discriminant analysis of the effect size revealed significant enrichment of taxa such as Burkholderiaceae and Ralstonia in the Cry1Ab rhizosphere, in contrast to the higher abundance of Chloroflexi in the WT. Functional predictions indicated consistent metabolic pathways across all treatments, suggesting strong ecological redundancy. This study demonstrates minimal impact on rhizosphere microbial communities in transgenic C. bungei plants. The Cry9-2 construct exhibited superior environmental stability, whereas the Cry1Ab construct caused only slight but ecologically acceptable shifts. These findings support the ecological safety of Bt-transgenic C. bungei and identify Cry9-2 as a particularly favorable candidate for forestry applications. This comparative evaluation of three Cry genes in a tree species provides a framework for future gene-specific biosafety assessments in woody plants.