文献类型：期刊文献

英文题名：Identification and functional characterization of ABCC transporters for Cd tolerance and accumulation in Sedum alfredii Hance

作者：Feng, Tongyu[1] He, Xuelian[1] Zhuo, Renying[1] Qiao, Guirong[1] Han, Xiaojiao[1] Qiu, Wenmin[1] Chi, Linfeng[2] Zhang, Dayi[3] Liu, Mingying[2]

第一作者：Feng, Tongyu

通信作者：Liu, MY[1];Zhang, DY[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Zhejiang Chinese Med Univ, Sch Basic Med Sci, Hangzhou 310053, Peoples R China;[3]Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000608975400040)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31870647) and the funding of Zhejiang Chinese Medical University (No. 2020ZR08). D.Z. also acknowledges the support of the Chinese Government's Thousand Talents Plan for Young Professionals.

语种：英文

摘要：Cd is one of the potential toxic elements (PTEs) exerting great threats on the environment and living organisms and arising extensive attentions worldwide. Sedum alfredii Hance, a Cd hyperaccumulator, is of great importance in studying the mechanisms of Cd hyperaccumulation and has potentials for phytoremediation. ATP-binding cassette sub-family C (ABCC) belongs to the ABC transporter family, which is deemed to closely associate with multiple physiological processes including cellular homeostasis, metal detoxification, and transport of metabolites. In the present work, ten ABCC proteins were identified in S. alfredii Hance, exhibiting uniform domain structure and divergently clustering with those from Arabidopsis. Tissue-specific expression analysis indicated that some SaABCC genes had significantly higher expression in roots (Sa23221 and Sa88F144), stems (Sa13F200 and Sa14F98) and leaves (Sa13F200). Co-expression network analysis using these five SaABCC genes as hub genes produced two clades harboring different edge genes. Transcriptional expression profiles responsive to Cd illustrated a dramatic elevation of Sa14F190 and Sa18F186 genes. Heterologous expression in a Cd-sensitive yeast cell line, we confirmed the functions of Sa14F190 gene encoding ABCC in Cd accumulation. Our study performed a comprehensive analysis of ABCCs in S. alfredii Hance, firstly mapped their tissue-specific expression patterns responsive to Cd stress, and characterized the roles of Sa14F190 genes in Cd accumulation.