文献类型：期刊文献

英文题名：Production of an epigenetic mutant population ofPopulus nigra: DNA methylation and phenotype analyses

作者：Zhong, Shanchen[1] Gao, Yanan[1] Lu, Junqian[1] Ding, Changjun[1] Su, Xiaohua[1] Zhang, Bingyu[1]

第一作者：Zhong, Shanchen

通信作者：Su, XH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2021

卷号：30

期号：2

起止页码：354-363

外文期刊名：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;Scopus(收录号：2-s2.0-85090848254);WOS:【SCI-EXPANDED(收录号:WOS:000568501700001)】；

基金：This research was supported by the National Natural Science Foundation of China (Grant No. 30770710).

语种：英文

外文关键词：Populus nigra; 5-AzaC; Plant regeneration; DNA methylation; MSAP; Phenotypic variation

摘要：5-Azacytidine (5-AzaC) causes hypomethylation of genomic DNA and induces phenotype variation in many plant species. Modulating the methylation status of cytosine by 5-AzaC to generate novel phenotypes in poplars have not been attempted. In this study, a population of 288 plants was regenerated from leaves ofPopulus nigraon medium supplemented with 100-1000 mu M 5-AzaC. The differentiation of leaves was delayed and the differentiation rate decreased as the 5-AzaC concentration increased. Compared with plants regenerated on 5-AzaC-free medium (control), the plants regenerated on high-5-AzaC medium (600-1000 mu M) had significantly higher DNA methylation levels and were shorter, whereas the plants regenerated on low-5-AzaC medium were unchanged; the plants regenerated on 100 mu M 5-AzaC medium had significant more leaves; the plants regenerated on 400 mu M 5-AzaC medium had significantly higher leaf chlorophyllband total chlorophyll contents, while the plants on 800 and 1000 mu M 5-AzaC medium had a significantly lower chlorophyllacontent; most photosynthetic parameters of the plants regenerated on 5-AzaC-treated medium decreased, except for the net photosynthetic rate (P-n) and transpiration rate (T-r) of plants on 100, 200, and 400 mu M medium, and the instant water utilization efficiency (WUEi) of plants on 1000 mu M medium. The superoxide dismutase activity of plants on >= 400 mu M 5-AzaC medium was significantly higher than in the control. Significant weak correlations were found between the methylation level of regenerants and plant height, chlorophyllacontent, P-n, T-r, stomatal conductance, intercellular CO(2)concentration, and WUEi, indicating these phenotypic changes were related to the methylation changes. Our results demonstrated the use of 5-AzaC at the regeneration stage induced epigenetic as well as phynotypic variations in poplar regenerants and generated plant materials for genetic breeding and epigenetic studies.