文献类型：期刊文献

英文题名：PagWOX11/12a activates PagCYP736A12 gene that facilitates salt tolerance in poplar

作者：Wang, Liu-Qiang[1,2] Wen, Shuang-Shuang[1] Wang, Rui[3] Wang, Chao[3] Gao, Bei[4] Lu, Meng-Zhu[1,2,5]

第一作者：Wang, Liu-Qiang;王留强

通信作者：Lu, MZ[1];Lu, MZ[2];Lu, MZ[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Peoples R China;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China;[4]Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China;[5]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou, Peoples R China

年份：2021

卷号：19

期号：11

起止页码：2249-2260

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000675086400001)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (No. CAFYBB2018QB002) and National Natural Science Foundation of China (No. 31600539 and 31971620).

语种：英文

外文关键词：cytochrome P450 monooxygenase gene; PagWOX11; 12a; root growth; ROS; salt stress

摘要：The WUSCHEL-related homeobox (WOX) transcription factors WOX11 and WOX12 regulate adventitious rooting and responses to stress. The underlying physiological and molecular regulatory mechanisms in salt stress tolerance remain largely unexplored. Here, we characterized the roles of PagWOX11/12a from 84K poplar (Populus alba x P. glandulosa) and the underlying regulatory mechanism in salt stress. PagWOX11/12a was strongly induced by salt stress in roots. Overexpression of PagWOX11/12a in poplar enhanced salt tolerance, as evident by the promotion of growth-related biomass. In contrast, salt-treated PagWOX11/12a dominant repression plants displayed reduced biomass growth. Under salt stress conditions, PagWOX11/12a-overexpressed lines showed higher reactive oxygen species (ROS) scavenging capacity and lower accumulation of hydrogen peroxide (H2O2) than non-transgenic 84K plants, whereas the suppressors displayed the opposite phenotype. In addition, PagWOX11/12a directly bound to the promoter region of PagCYP736A12 and regulated PagCYP736A12 expression. The activated PagCYP736A12 could enhance ROS scavenging, thus reducing H2O2 levels in roots under salt stress in PagWOX11/12a-overexpressed poplars. The collective results support the important role of PagWOX11/12a in salt acclimation of poplar trees, indicating that PagWOX11/12a enhances salt tolerance through modulation of ROS scavenging by directly regulating PagCYP736A12 expression in poplar.