文献类型：期刊文献

英文题名：Ectopic Expression of Litsea cubeba LcMADS20 Modifies Silique Architecture

作者：Jiao, Yulian[1,2] Yin, Hengfu[1,2] Chen, Yicun[1,2] Gao, Ming[1,2] Wu, Liwen[1,2] Wang, Yangdong[1,2]

第一作者：Jiao, Yulian

通信作者：Wang, YD[1];Wang, YD[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 10091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2019

卷号：9

期号：12

起止页码：4139-4147

外文期刊名：G3-GENES GENOMES GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000511967400022)】；

基金：The work was financially supported by the Science and Technology Major Program on Agricultural New Variety Breeding of Zhejiang, China (no. 2016C02056); "Ten thousand people plan" Science and Technology Innovation Leading Talent of Zhejiang, China (no. 2018R52006); and a National Key R&D Program of China grant (no. 2017YFD0600704) awarded to Y.-C.C.

语种：英文

外文关键词：Lauraceae; mountain pepper; AGAMOUS-like genes; ectopic expression; fruit

摘要：Litsea cubeba (Lour.) Pers. (mountain pepper, Lauraceae) is an important woody essential oil crop that produces fragrant oils in its fruits, especially in its peels. Identification of genes involved in the regulation of fruits and peel architecture is of economic significance for L. cubeba industry. It has been well known that the MADS-box genes are essential transcription factors that control flowers and fruits development. Here, we obtained 33 MADS-box genes first from the RNA-seq data in L. cubeba, and 27 of these genes were of the MIKC-type. LcMADS20, an AGAMOUS-like gene, was highly expressed in the developing stages of fruits, particularly at 85 days after full bloom. The ectopic expression of LcMADS20 in Arabidopsis resulted in not only curved leaves, early flowering and early full-opened inflorescences, but also shorter siliques and decreased percentage of peel thickness. Moreover, in the LcMADS20 transgenic Arabidopsis, the expression modes of several intrinsic ABC model class genes were influenced, among which the expression of FUL was significantly reduced and AP3, AG, and STK were significantly increased. This study systematically analyzed the MADS-box genes in L. cubeba at the transcriptional level and showed that LcMADS20 plays important roles in the regulation of fruit architecture.