文献类型：期刊文献

中文题名：菌根真菌对3种兰花幼苗生长作用研究

英文题名：Effect of Mycorrhizal Fungi on the Plantlets Growth of Three Species of Orchid

作者：杨前宇[1] 何聪芬[2] 梁立雄[1] 王涛[1] 李潞滨[1] 刘蕾[1]

第一作者：杨前宇

机构：[1]中国林业科学研究院林业研究所/林木遗传育种国家重点实验室/国家林业局林木培育重点实验室;[2]北京工商大学/北京市植物资源研究开发重点实验室

年份：2019

卷号：0

期号：4

起止页码：687-695

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：北京市植物资源研究开发重点实验室开放课题(PRRD-2016-ZD1)

语种：中文

中文关键词：兰科植物;菌根真菌;共生关系;专一性

外文关键词：Orchidaceae;mycorrhiza fungi;symbiosis;specificity

分类号：S682.31

摘要：菌根真菌在兰科植物生活史中具有重要作用。为获得促进兰科植物生长的共生真菌,阐明菌根真菌对兰科植物的作用,本研究对从野生建兰中分离获得的3株共生真菌进行形态学和系统进化分析,并将3株真菌与建兰、硬叶兰和铁皮石斛幼苗在不同浓度燕麦琼脂培养基(OMA)条件下共培养,进行比较研究。结果表明,3株真菌分属于胶膜菌属(Tsc)、角担菌属(Cej3)和蜡壳菌属(Sec),且菌丝和菌落特征存在明显差异;系统进化分析发现,Tsc菌株与Cej3菌株亲缘关系较近,与Sec菌株亲缘关系较远;与兰科植物共培养结果表明,Tsc菌株可在全部OMA浓度条件下与3种兰科植物幼苗建立共生关系,且定殖率均为100%; Cej3菌株和Sec菌株与3种兰科植物在不同OMA浓度条件下定殖时间及定殖率存在差异,且具有一定的专一性;与对照组相比,与真菌共生的兰科植物幼苗的平均鲜重、干重、苗高、根长增长率均明显提高。本研究结果为深入研究兰科植物与真菌共生的分子机制奠定了一定的理论基础,并为兰花种质资源的保护提供了参考。
Mycorrhizal fungi play an important role in the life history of orchid plants. In order to obtain the mycorrhizal fungi promoting the growth of orchid plants,understand the effect of mycorrhizal fungi on orchid plants,three strains isolated from Cymbidium ensifolium were identified by molecular and morphological analysis,were co-cultivated with three species of orchid plantlets on different concentration medium. The results showed that three strains were identified as Tulasnella sp.( Tsc),Ceratobasidium sp.( Cej3) and Sebacina sp.( Sec) using the ITS-rDNA sequence analysis. The mycelium and colonial morphological characteristics of three strains were obvious different. Phylogenetic analysis showed that strain Tsc had closer relationship with Cej3 than with Sec. Strain Tsc could symbiosis with three orchid species on all OMA concentrations,and the colonization rate were 100%;the colonization time and rate of strain Cej3 and Sec with three orchid species were different on different OMA concentrations. Analysis found that the fresh weight,dry weight,plantlets height and root length of treatment group were increased significantly by mycorrhizal fungi inoculation compared with control group. These results will lay the basis for further studying the molecular mechanism of the symbiosis between orchid and mycorrhizal fungi and provide a reference for orchid plants conservation.