文献类型：期刊文献

英文题名：Molecular genetic variability of Juglans regia L. and Juglans sigillata D. as revealed by fluorescent amplified-fragment length polymorphism

作者：Ma, Qing-guo[1] Zhang, Jun-pei[1] Pei, Dong[1]

第一作者：马庆国

通信作者：Pei, Dong

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China

年份：2020

卷号：34

期号：1

起止页码：725-731

外文期刊名：Biotechnology and Biotechnological Equipment

收录：EI(收录号：20203309051364);Scopus(收录号：2-s2.0-85089264367)

语种：英文

外文关键词：Genes - Polymorphism - Biodiversity

摘要：Juglans regia L. and J. sigillata D. have a long cultivation history in China. With the purpose of detecting interspecific and intraspecific genetic variation, 35 clonal cultivars of J. regia, J. sigillata, J. sigillata×J. regia and J. hopeiensis Hu. (J. mandshurica Maxim. ×J. regia) were selected from a broad geographic range and applied in amplified-fragment length polymorphism (AFLP) analysis. Clear-cut AFLP profiles were produced using 9 EcoR I/Mse I primer combinations. As for J. regia, J. sigillata and their hybrids, the percentages of polymorphic bands (PPBs) averaged 93.86%, 93.94% and 60.46%; the observed number of alleles (Na) averaged 1.5370, 1.6389 and 1.2593; the effective number of alleles (Ne) averaged 1.2582, 1.2543 and 1.1646; Nei's gene diversity (H) scored 0.1560, 0.1556 and 0.0951; Shannon’s information index (I) averaged 0.2402, 0.2459 and 0.1414, respectively. These results implied a moderate level of genetic diversity within the investigated species. The total gene diversity (Ht = 0.1596) can be distributed into intra- (Hs = 0.1356) and inter- (Dst = 0.0240) species gene diversity. The proportion of the distributed genetic diversity among populations (Gst) was 0.1505, indicating that only 15% of the total gene diversity was attributed to differences between species. The factorial correspondence analysis (FCA) and unweighted pair-group method of arithmetic averages (UPGMA) analysis revealed a close genetic distance between these two species. ? 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.