文献类型：期刊文献

中文题名：草莓愈伤组织再生能力与活性氧代谢水平相关性研究

英文题名：The Possible Relationship between the Regeneration Capacity and Reactive Oxygen Species in the Strawberry Calli

作者：田敏[1] 韩凝[2] 边红武[2] 朱睦元[2]

第一作者：田敏

机构：[1]中国林业科学研究院亚热带林业研究所;[2]浙江大学生命科学学院

年份：2004

卷号：31

期号：3

起止页码：372-374

中文期刊名：园艺学报

外文期刊名：Acta Horticulturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：草莓;活性氧;抗氧化酶;再生能力;愈伤组织

外文关键词：Strawberry;Reactive oxygen species;Antioxidant enzymes;Regeneration capacity;Callus

分类号：S668.4

摘要：草莓外植体在不同组合培养基上诱导产生 3类愈伤组织 ,Ⅰ型愈伤组织的再生能力较低 ,Ⅱ和Ⅲ型愈伤组织具有较高的再生能力。Ⅱ和Ⅲ型初生愈伤组织H2 O2 的含量分别是Ⅰ型的 5倍和 9倍 ,O·2的释放速率是其 3倍和 4倍 ,并且具有较高的抗氧化酶活性。在继代培养过程中 ,愈伤组织的形态发生能力下降 ,Ⅰ型愈伤组织逐渐失去再生能力 ,同时所有类型愈伤组织中H2 O2 含量缓慢上升 ,O·2 释放速率较快增长 。
Three types of callus,which present different regeneration capacity,were established in the tissue culture of strawberry.The Ⅱ and Ⅲ types of callus possessed higher regeneration capacity while Ⅰ type of callus had much lower.In the freshly induced calli,the Ⅱ and Ⅲ types of callus had 5 fold and 9 fold higher contents of intracellular H 2O 2,and 3 fold and 4 fold higher level of O [BHDFG1.5mm,WK1.5mmW]· 2,compared with the Ⅰ type of callus, respectively .Also,the calli Ⅱ and Ⅲ had much higher activities of antioxidant enzymes than the callus Ⅰ.During the subculture,H 2O 2 level increased gradually and O [BHDFG1.5mm,WK1.5mmW]· 2 rapidly,while the activities of antioxidant enzymes gradually declined.The ability of expression of totipotency in calli decreased,especially the callus Ⅰ lost the ability after 12 week subculture. The results indicated that the reactive oxygen species might play a dual role in the regeneration of strawberry calli.On the one hand,a certain level of ROS may have a positive effect on the regeneration,and on the other hand, high level of ROS is inhibitory for the expression of totipotency in calli.