文献类型：期刊文献

中文题名：转Bt基因和蛋白酶抑制剂基因杨树抗虫性的研究

英文题名：STUDY ON INSECT RESISTANT TRANSGENIC POPLAR PLANTS CONTAINING BOTH BT AND PI GENE

作者：李明亮[1] 张辉[1] 胡建军[1] 韩一凡[1] 田颖川[2]

第一作者：李明亮

机构：[1]中国林业科学研究院林业研究所;[2]中国科学院微生物研究所

年份：2000

卷号：36

期号：2

起止页码：93-97

中文期刊名：林业科学

外文期刊名：SCIENTIA SILVAE SINICAE

收录：CSTPCD;;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：欧洲黑杨;Bt基因;PI基因;转基因植物;抗虫性

外文关键词：Populus nigra , Bt gene, Proteinase inhibitor gene, Insect resistance

分类号：S792.110.4;S722.36

摘要：虫害是杨树造林生产中所面临的一大难题。通过农杆菌介导 ,将经过人工改造的苏云金杆菌杀虫结晶蛋白 (Bt)基因转化杨树 ,得到杨树再生植株 ,然后 ,再将蛋白酶抑制剂基因 (PI)导入已含Bt基因的转基因杨树 ,经PCR检测和Southern杂交分析证明 ,最终获得既含有Bt基因又含有蛋白酶抑制剂基因的转基因杨树植株。利用这种杨树叶片饲喂舞毒蛾幼虫的杀虫试验结果表明 ,转基因杨树具有明显的杀虫活性 ,同时表明含有Bt基因和蛋白酶抑制剂基因双基因的植株其抗虫能力明显高于仅含单一Bt基因的植株。为探索培育高抗虫性杨树开辟了一条新途径。
Poplars was seriously attacked by insects usually in many areas and insect resistant poplars are expected in forest production. In the experiment, the modified Bacillus thuringiensis (Bt) δ endotoxin gene and proteinase inhibitor(PI)gene were contransformed to the explants of Populus nigra mediated by Agrobacterium tumefaciens. The integration of Bacillus thuringiensis gene and proteinase inhibitor(PI)gene were confirmed by PCR analysis and Southern hybridization. The results of bioassay showed that the transgenic plants had toxicity to the larvae of Lymantria dispar L., and that the transgenic plants containing Bt gene and PI gene had enhanced toxicity to larvae by comparison with plants containing only Bt gene.