文献类型：期刊文献

英文题名：Effects of Different Donor Ages on the Growth of Cutting Seedlings Propagated from Ancient Platycladus orientalis

作者：Dong, Yao[1] Guo, Wei[2] Xiao, Wenfa[1] Liu, Jianfeng[3] Jia, Zirui[3] Zhao, Xiulian[3] Jiang, Zeping[1] Chang, Ermei[3]

第一作者：Dong, Yao

通信作者：Chang, ER[1]

机构：[1]Chinese Acad Forestry, Ecol & Nat Conservat Inst, Key Lab Forest Ecol & Environm, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Taishan Acad Forestry Sci, Tai An 271000, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry & G, Beijing 100091, Peoples R China

年份：2023

卷号：12

期号：9

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000987327000001)】；

基金：This work was supported by the State Natural Science Fund projects (31870664), and the open fund of the State Key Laboratory of Subtropical Silviculture (SKLSS-KF2022-09). The National Non-Profit Research Institutions of the Chinese Academy of Forestry [Grant numbers: CAFYBB2020ZD002-4].

语种：英文

外文关键词：Platycladus orientalis; transcriptome; growth; cutting seedlings; ancient tree

摘要：The effects of tree age on the growth of cutting seedlings propagated from ancient trees have been an important issue in plant breeding and cultivation. In order to understand seedling growth and stress resistance stability, phenotypic measurements, physiological assays, and high-throughput transcriptome sequencing were performed on sown seedlings propagated from 5-year-old donors and cutting seedlings propagated from 5-, 300-, and 700-year-old Platycladus orientalis donors. In this study, the growth of cutting seedlings propagated from ancient trees was significantly slower; the soluble sugar and chlorophyll contents gradually decreased with the increase in the age of donors, and the flavonoid and total phenolic contents of sown seedlings were higher than those of cutting seedlings. Enrichment analysis of differential genes showed that plant hormone signal transduction, the plant-pathogen interaction, and the flavone and flavonol biosynthesis pathways were significantly up-regulated with the increasing age of cutting seedlings propagated from 300- and 700-year-old donors. A total of 104,764 differentially expressed genes were calculated using weighted gene co-expression network analysis, and 8 gene modules were obtained. Further, 10 hub genes in the blue module were identified, which revealed that the expression levels of JAZ, FLS, RPM1/RPS3, CML, and RPS2 increased with the increase in tree age. The results demonstrated that the age of the donors seriously affected the growth of P. orientalis cutting seedlings and that cutting propagation can preserve the resistance of ancient trees. The results of this study provide important insights into the effects of age on asexually propagated seedlings, reveal potential molecular mechanisms, and contribute to an improvement in the level of breeding and conservation of ancient germplasm resources of P. orientalis trees.