文献类型：期刊文献

英文题名：Comprehensive analysis and identification of the WOX gene family in Schima superba and the key gene SsuWOX1 for enhancing callus regeneration capacity

作者：Jia, Yuanting[1,2,3] Lin, Zihang[1,2,3] He, Haixin[1,2,3] Zhou, Zhichun[2,3] Gao, Kai[2,3] Du, Kejiu[1] Zhang, Rui[2,3]

第一作者：Jia, Yuanting

通信作者：Du, KJ[1];Gao, K[2];Zhang, R[2];Gao, K[3];Zhang, R[3]

机构：[1]Agr Univ Hebei, Baoding 071000, Hebei, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[3]Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Zhejiang, Peoples R China

年份：2025

卷号：25

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-105000554396);WOS:【SCI-EXPANDED(收录号:WOS:001449226300006)】；

基金：We are acknowledging the Research Institute of Subtropical Forestry, Chinese Academy of Forestry for providing experimental facilities and other necessary materials for this study. We thank all colleagues for their fruitful discussions and technical assistance. We are very appreciative of the critical evaluation of the manuscript by the editors and reviewers, and for their constructive suggestions for improving the manuscript.

语种：英文

外文关键词：WOXs gene family; Systematic classification; Genetic structure; Egeneration; Schima superba

摘要：This study conducted a comprehensive analysis of the SsuWOX gene family in Schima superba, elucidating its role in plant growth and stress response mechanisms. The genome contains 15 WOX genes primarily encoding nuclear proteins unevenly distributed across 18 chromosomes. Phylogenetic classification grouped these genes into three distinct subfamilies, with members in each subfamily showing conserved gene structures. Interaction network analysis and cis-regulatory element characterization revealed that SsuWOX gene expression is influenced by hormones and various abiotic stresses. Tissue-specific expression profiles showed six genes exhibiting spatial specificity with significant expression level variations across developmental stages. Notably, SsuWOX1 overexpression in callus tissue significantly elevated CLAVATA3 (CLV3) expression levels. CLV3, a crucial small peptide signaling molecule, primarily regulates stem cell maintenance and differentiation in the shoot apical meristem (SAM). Transgenic callus cells displayed bud-like cell characteristics, including increased cell density and organized spatial arrangement. These findings establish a foundation for functional characterization of SsuWOX1 and provide insights into its regulatory mechanisms in plant development.