文献类型：期刊文献

英文题名：Identification and expression analysis of CCCH gene family and screening of key low temperature stress response gene CbuC3H24 and CbuC3H58 in Catalpa bungei

作者：Bao, Pingan[1,2] Sun, Jingshuang[1] Qu, Guanzheng[2] Yan, Maolin[3] Cheng, Shiping[4] Ma, Wenjun[5] Wang, Junhui[5] Hu, Ruiyang[1]

第一作者：Bao, Pingan

通信作者：Hu, RY[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, State Key Lab Tree Genet & Breeding, Natl Permanent Sci Res Base Warm Temperate Zone Fo, Beijing 102300, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[3]Inner Mongolia Acad Forestry, Hohhot 010010, Peoples R China;[4]Pingdingshan Univ, Henan Key Lab Germplasm Innovat & Utilizat Ecoecon, Pingdingshan 467000, Henan, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2024

卷号：25

期号：1

外文期刊名：BMC GENOMICS

收录：;Scopus(收录号：2-s2.0-85200982340);WOS:【SCI-EXPANDED(收录号:WOS:001288935700001)】；

基金：We express our gratitude to the anonymous reviewers for their critical comments on the manuscript.

语种：英文

外文关键词：Catalpa bungei; CCCH zinc finger gene; Gene family; Low temperature stress; Gene expression

摘要：Catalpa bungei, a tree indigenous to China, is renowned for its superior timber quality and as an ornamental in horticulture. To promote the cultivation of C. bungei in cold regions and expand its distribution, enhancing its cold tolerance is essential. The CCCH gene family is widely involved in plant growth, development, and expression under stress conditions, including low-temperature stress. However, a comprehensive identification and analysis of these genes have not yet been conducted. This study aims to identify key cold-tolerance-related genes within the CCCH gene family of C. bungei, providing the necessary theoretical support for its expansion in cold regions. In this study, 61 CCCH genes within C. bungei were identified and characterized. Phylogenetic assessment divided these genes into 9 subfamilies, with 55 members mapped across 16 chromosomes. The analysis of gene structures and protein motifs indicated that members within the same subfamily shared similar exon/intron distribution and motif patterns, supporting the phylogenetic classification. Collinearity analysis suggested that segmental duplications have played a significant role in the expansion of the C. bungei CCCH gene family. Notably, RNA sequencing analysis under 4 degrees C cold stress conditions identified CbuC3H24 and CbuC3H58 as exhibiting the most significant responses, highlighting their importance within the CCCH zinc finger family in response to cold stress. The findings of this study lay a theoretical foundation for further exploring the mechanisms of cold tolerance in C. bungei, providing crucial insights for its cultivation in cold regions.