文献类型：期刊文献

英文题名：Genome-wide analysis of the Catalpa bungei caffeic acid O-methyltransferase (COMT) gene family: identification and expression profiles in normal, tension, and opposite wood

作者：Lu, Nan[1] Ma, Wenjun[1] Han, Donghua[2] Liu, Ying[3] Wang, Zhi[1] Wang, Nan[1] Yang, Guijuan[1] Qu, Guanzheng[4] Wang, Qiuxia[5] Zhao, Kun[6] Wang, Junhui[1]

第一作者：卢楠

通信作者：Ma, WJ[1];Wang, JH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Coll Landscape Architecture, Nanjing, Jiangsu, Peoples R China;[3]Northwest A&F Univ, Coll Forestry, Yangling, Shaanxi, Peoples R China;[4]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Heilongjiang, Peoples R China;[5]Nanyang Res Inst Forestry, Nanyang, Peoples R China;[6]Luoyang Acad Agr & Forestry, Luoyang, Peoples R China

年份：2019

卷号：7

期号：3

外文期刊名：PEERJ

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000461256200001)】；

基金：This research was funded by Forestry Industry Research Special Funds for Public Welfare Projects (No. 201404101). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Tension wood; Catalpa bungei; Genome-wide; COMTs

摘要：Caffeic acid O-methyltransferase (COMT) is an important protein that participates in lignin synthesis and is associated with the ratio of G-/S-type lignin in plants. COMTs are associated with the wood properties of forest trees; however, little known about the COMT family in Catalpa bungei, a valuable timber tree species in China. We performed a comprehensive analysis of COMT genes in the C. bungei genome by describing the gene structure and phylogenetic relationships of each family member using bioinformatics-based methods. A total of 23 putative COMT genes were identified using the conserved domain sequences and amino acid sequences of COMTs from Arabidopsis thaliana and Populus trichocarpa as probes. Phylogenetic analysis showed that 23 CbuCOMTs can be divided into three groups based on their structural characteristics; five conserved domains were found in the COMT family. Promoter analysis indicated that the CbuCOMT promoters included various cis-acting elements related to growth and development. Real-time quantitative polymerase chain reaction (PCR) analysis showed differential expression among CbuCOMTs. CbuCOMT2, 7, 8, 9, 10, 12, 13, 14, 21, and 23 were mainly expressed in xylem. Only CbuCOMT23 was significantly downregulated in tension wood and upregulated in opposite wood compared to normal wood. Our study provides new information about the CbuCOMT gene family and will facilitate functional characterisation in further research.