文献类型：期刊文献

中文题名：印度黄檀叶多酚提取工艺优化及抗氧化活性研究

英文题名：Optimization of Extraction Process and Antioxidant Activity of Polyphenols from Leaves of Dalbergia sissoo(Roxb.)

作者：邓疆[1] 王有琼[1] 石雷[1]

第一作者：邓疆

机构：[1]中国林业科学研究院资源昆虫研究所

年份：2018

卷号：31

期号：6

起止页码：161-167

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家重点研发计划项目子课题资助:印度黄檀高效培育技术研究(2016YFD0600605)

语种：中文

中文关键词：印度黄檀;叶;多酚;微波萃取;抗氧化活性

外文关键词：Dalbergia sissoo;polyphenols;microwave extraction;antioxidant activity

分类号：S759.3

摘要：目的]印度黄檀叶含有多酚及类黄酮物质,研究印度黄檀叶多酚及其抗氧化活性,可为其利用提供依据。[方法]以印度黄檀叶为原料,乙醇为提取液,经单因素实验与正交试验设计,检测在不同乙醇浓度、提取时间、提取温度及超声功率120 w时3个因素进行响应面优化试验,确定印度黄檀多酚的提取工艺;同时,鉴定印度黄檀叶乙醇-水提取液对DPPH-自由基的清除能力。[结果]低浓度印度黄檀叶多酚能发挥更强的抗氧化能力,其提取液对清除DPPH自由基的半数抑制质量浓度(IC_(50))约为3.2 mg·L^(-1),略大于Vc的2.5 mg·L^(-1);不过,其还原能力略低于Vc。[结论]印度黄檀叶内富含多酚类物质,具有很强的体外抗氧化活性,可作为天然抗氧化植物资源开发利用。
[Objective]The leaves of Dalbergia sissoo(Roxb.)contain polyphenols and flavonoids.Polyphenols and flavonoids are scavengers of free radicals in organisms,preventing the toxic effects of free radicals on the body and inhibiting lipid peroxidation,i.e.antioxidant activity.Therefore,understanding of polyphenols extraction form leaves and its antioxidant activity in vitro could provide theoretical basis of utilization of leaves of D.sissoo.[Method]Ethanol was used as the extract of polyphenols in leaves of D.sissoo,and then single factor experiment and orthogonal experiment were used to determine the extraction process of polyphenols.At the same time,the removal capacity of DPPH-free radical of ethanol-water extract of leaves of D.sissoo was identified.[Result]Low-concentration of polyphenol solution of leaves of D.sissoo had strong antioxidant activity.The concentration of polyphenol solution for inhibitory half number of DPPH free radical(IC 50)was about 3.2 mg·L^-1,much higher than concentration 2.5 mg·L^-1 of Vc,but its reducing ability was slightly lower than the Vc.[Conclusion]Leaves of D.sissoo are rich in polyphenols and have strong antioxidant activity,which can be used as natural antioxidant plant resources for development and utilization.