文献类型：期刊文献

中文题名：Single-cell RNA sequencing reveals a high-resolution cell atlas of xylem in Populus

作者：Hui Li[1] Xinren Dai[1] Xiong Huang[1,2] Mengxuan Xu[1] Qiao Wang[1,2] Xiaojing Yan[1] Ronald R.Sederoff[3] Quanzi Li[1]

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China;[2]Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;[3]Forest Biotechnology Group,Department of Forestry and Environmental Resources,North Carolina State University,Raleigh,NC 27695,USA

年份：2021

卷号：63

期号：11

起止页码：1906-1921

中文期刊名：植物学报：英文版

外文期刊名：Journal of Integrative Plant Biology

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：This work was supported by grants from Fundamental Research Funds of Chinese Academy of Forestry(CAFYBB2018ZY001-5 and CAFYBB2017ZY001);the National Natural Science Foundation of China(31670667)。

语种：英文

中文关键词：differentiating trajectory;differentiating xylem;marker genes;Populus alba×Populus glandulosa;single-cell RNA-seq;wood formation

分类号：S792.11

摘要：High-throughputsingle-cellRNAsequencing(sc RNA-seq) has advantages over traditional RNA-seq to explore spatiotemporal information on gene dynamic expressions in heterogenous tissues. We performed Drop-seq, a method for the dropwise sequestration of single cells for sequencing, on protoplasts from the differentiating xylem of Populus alba × Populus glandulosa. The sc RNA-seq profiled9,798 cells, which were grouped into 12 clusters.Through characterization of differentially expressed genes in each cluster and RNA in situ hybridizations,we identified vessel cells, fiber cells, ray parenchyma cells and xylem precursor cells. Diffusion pseudotime analyses revealed the differentiating trajectory of vessels, fiber cells and ray parenchyma cells and indicated a different differentiation process between vessels and fiber cells, and a similar differentiation process between fiber cells and ray parenchyma cells. We identified marker genes for each cell type(cluster) and key candidate regulators during developmental stages of xylem cell differentiation. Our study generates a high-resolution expression atlas of wood formation at the single cell level and provides valuable information on wood formation.