文献类型：期刊文献

英文题名：DEVELOPMENT AND CHARACTERISATION OF EST-SSR MARKERS FOR GENETIC ANALYSIS OF CASUARINA SPECIES

作者：Li, Z.[1] Zhang, Y.[1] Hu, P.[2] Zhong, C. L.[1] Wei, Y. C.[1] Meng, J. X.[1] Wang, Y. J.[1] Bush, D.[3]

通信作者：Zhang, Y[1]|[a00051856db9904c755bf]张勇;

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[3]CSIRO, Australian Tree Seed Ctr, GPO Box 1600, Canberra, ACT 2601, Australia

年份：2021

卷号：33

期号：4

起止页码：425-434

外文期刊名：JOURNAL OF TROPICAL FOREST SCIENCE

收录：;EI(收录号：20220111410930);Scopus(收录号：2-s2.0-85121927274);WOS:【SCI-EXPANDED(收录号:WOS:000711973400005)】；

基金：This study was financially supported by the National Natural Science Foundation of China (Grant No. 31770716) , and the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018ZB003) .

语种：英文

外文关键词：Mendelian inheritance; cross-species transferability; parentage analysis

摘要：Simple sequence repeats (SSR) markers have greater advantages relative to other molecular markers in genetic analysis due to their Mendelian inheritance and co-dominance expression features. Numerous expressed sequence tags (EST) of genus Casuarina, now available from public databases, make it possible to develop EST-SSR markers with high efficiency at low cost. From the analysis of 12,063 EST derived from casuarinas, 367 SSR loci were identified from 352 unigenes, and the distribution frequency of SSR loci was 3.0%. The di- and trinucleotide accounted for 57.77 and 34.06% respectively. They comprised overwhelming majority in five types of repeat motifs of SSR, and AG/CT was the most predominant motif type of SSR. A total of 79 primer pairs were designed based on the non-redundant EST-SSR sequences, but only 21 were successfully amplified and generated polymorphic SSR products in four different Casuarina species and eight clones of C. equisetifolia. Eight primer pairs out of the 21 were applied for paternity analyses of open-pollinated progenies of one female individual of C. equisetifolia. At 95% confidence interval, 76 male individuals were identified as pollen parents of 262 out of 461 progenies, but at 80% confidence interval, 84 male individuals were identified as father trees of 328 out of the 461 progenies.