文献类型：期刊文献

英文题名：Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana

作者：Liu, Juanjuan[1,2] Zeng, Yanfei[1,2] Yan, Pengcheng[3] He, Caiyun[1,2] Zhang, Jianguo[1,2]

第一作者：刘娟娟;Liu, Juanjuan

通信作者：Zhang, JG[1];Zhang, JG[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China;[3]Beijing Comp Ctr, Beijing Key Lab Cloud Comp Key Technol & Applicat, Beijing 100094, Peoples R China

年份：2017

卷号：8

期号：12

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000419212400020)】；

基金：This work was supported by National Nonprofit Institute Research Grants from the Research Institute of Forestry, Chinese Academy of Forestry (RIF2012-03), the National Natural Science Foundation of China (31700556) and Fundamental Research Funds of CAF (CAFYBB2017QA002). We thank Prof. IC Bruce (Peking University) for critical reading of the manuscript.

语种：英文

外文关键词：phytohormone; gibberellin; auxin; weeping; transcription factor; Salix matsudana

摘要：Salix matsudana is a large and rapidly-growing tree, with erect or spreading branchlets (upright willow). However, S. matsudana var. pseudomatsudana is one of the varietas, with pendulous branchlets (weeping willow). It has high ornamental value for its graceful pendulous branches. In order to study the molecular basis for this weeping trait, leaves and stems collected at different developmental stages were analyzed using RNA-seq coupled with digital gene expression. Although weeping trees are used worldwide as landscape plants, little is known about the genes that control weeping. Our growth results indicated that branches in weeping willow developed and elongated throughout all developmental stages, but branches in upright willow grew rapidly in the initial stages and then grew slowly and began shoot branching in the middle stages. A total of 613 hormone-related genes were differentially expressed in willow development. Among these, genes associated with auxin and gibberellin (GA) were highly likely to be responsible for the weeping trait, and genes associated with auxin and ethylene probably play crucial roles in shoot elongation. The genes with differential expression patterns were used to construct a network that regulated stem development, and auxin-related genes were identified as hub genes in the network in the weeping willow. Our results suggest an important role of gibberellin and auxin in regulating the weeping trait in Salix matsudana. This is the first report on the molecular aspects of hormonal effects on weeping trait in willow using transcriptomics and helps in dissecting the molecular mechanisms by which the weeping trait is controlled.