文献类型：期刊文献

中文题名：Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch

作者：Kuipeng Li[1,2] Hua Han[1] Yunhui Xie[1] Xiaomei Sun[1]

第一作者：Kuipeng Li

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing,100091,China;[2]Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation&Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of China,Guangxi Forestry Research Institute,Nanning,530002,China

年份：2021

卷号：90

期号：2

起止页码：583-593

中文期刊名：国际实验植物学杂志（英文）

外文期刊名：Phyton-International Journal of Experimental Botany

收录：Scopus;PubMed

基金：This research was funded by The National Key Research and Development Program of China(Project No.2017YFD0600401).

语种：英文

中文关键词：Hybrid larch;adventitious root;orthogonal test;variance analysis

分类号：S71

摘要：Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch(Larix spp.).We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots-activated carbon,vitamin B1,sucrose,glycine,glutamic acid(Glu),cysteine,pH,and induction time in the dark and indole-3-butyric acid(IBA).Variance analysis showed that the effects of sucrose concentration on rooting rate,survival rate,and average root number,pH on the average number of roots;and Glu on survival rate were all significant(p<0.05)when compared to the mock treatments.Among all of the 45 treatments,the highest rooting rate was 90%,the highest survival rate was 100%,and the highest average root number was 11.8.The theoretically optimal procedure of in vitro root induction of hybrid larch shoots is shoot induction on L9 modified medium(pH 6.7)containing 2 mg.L^(-1)naphthalene acetic acid,0.25 mg.L^(-1)IBA,1/3 macroelements,7.5 g.L^(-1)sucrose,and 5 mg.L^(-1)Glu for 8 days,and then root induction on L9 modified medium without auxin.