文献类型：期刊文献

英文题名：Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

作者：Wu, Qikui[1,2,3] Chen, Hong[1] Zhang, Zihan[1,4] Chen, Chen[1] Yu, Fangyuan[1] Guy, Robert D.[3]

第一作者：Wu, Qikui

通信作者：Yu, FY[1];Guy, RD[2]

机构：[1]Nanjing Forestry Univ, Coll Forest Sci, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing, Peoples R China;[2]Shandong Agr Univ, Coll Forestry, State Forestry & Grassland Adm Key Lab Silvicultur, Tai An, Peoples R China;[3]Univ British Columbia, Fac Forestry, Dept Forest & Conservat Sci, Vancouver, BC, Canada;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000811929800001)】；

基金：This work was supported by the Doctorate Fellowship Foundation of Nanjing Forestry University, Joint Research Project Based on Cooperative Program for Bachelor of Science in Forestry by Nanjing Forestry University and University of British Columbia, National Natural Science Foundation of China (3197140894), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). The funders had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

语种：英文

外文关键词：fruit shading; kernel development; carbon partitioning; oil biosynthesis; differentially expressed proteins

摘要：Styrax tonkinensis has great potential as a biofuel feedstock source having industrial oilseeds with excellent fatty acids (FAs) composition and good fuel properties. Photosynthesis in the developing pericarp could affect the carbon distribution in kernel. During kernel development, more carbon sources are allocated to starch rather than lipid, when the pericarp photosynthesis is reduced by fruit shading treatment. After shading the fruits at 50 days after flowering (DAF), samples of shaded fruit (FSK) and controls (CK) were collected at 80 DAF and analyzed using the proteomic method. We identified 3,181 proteins, of which 277 were differentially expressed proteins, all downregulated in the FSK group. There were 56 proteins found involved in carbohydrate metabolism and lipid biosynthesis leading to oil accumulation with their iTRAQ ratios of FSK/CK ranging from 0.7123 to 1.1075. According to the qRT-PCR analyses, the key genes related to FA and triacylglycerol (TAG) biosynthesis were significantly downregulated between 60 and 90 DAF especially at 80 DAF, while the key genes involved in starch biosynthesis and FA desaturase had no significant difference between the two groups at 80 DAF. Fruit shading is a negative treatment for lipid accumulation but not starch accumulation by restraining enzymic protein expression involved in FA and TAG biosynthesis during S. tonkinensis kernel development.