文献类型：期刊文献

英文题名：Calcium signaling mediated by glutamate receptor-like protein PagGLR3.3 is involved in tension wood induction in poplar

作者：An, Yi[1] Qu, Mei-Qiao[1] Geng, Ya[1] Jiao, Xue[1] Song, Xue-Qin[2] Zhao, Shu-Tang[2] Han, Xiao[1] Huang, Li-Chao[1] Zhang, Jin[1] Wang, Jie-Hua[1] Gerttula, Suzanne[3] Groover, Andrew[3] Lu, Meng-Zhu[1]

第一作者：An, Yi

通信作者：Lu, MZ[1]

机构：[1]Zhejiang A&F Univ, Natl Key Lab Dev & Utilizat Forest Food Resources, Zhejiang Key Lab Forest Genet & Breeding, Plant Cell Wall Res Ctr,Coll Forestry & Biotechnol, Hangzhou 311300, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]US Forest Serv, Northern Res Stn, Burlington, VT 05405 USA

年份：2026

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-105029006661);WOS:【SCI-EXPANDED(收录号:WOS:001676259100001)】；

基金：This study was supported by the National Key Research and Development Program of China "International Cooperation in Science and Technology Innovation between Governments (2019YFE0119100)"; the National Natural Science Foundation of China (NO. 32371902); and the research foundation of Zhejiang A&F University (2018FR013).

语种：英文

外文关键词：Ca2+; gravistimulation; O2-; PagGLR3.3; Populus; tension wood

摘要：Tension wood (TW), a type of reaction wood that develops in angiosperm trees in response to gravistimulation, serves as an ideal model for investigating the regulatory mechanisms underlying xylem cell differentiation and cell wall deposition. The initial biological signals that induce the formation of reaction wood in response to gravitational stimuli remain poorly understood. In this study, we utilized pharmacological and genetic approaches to modulate Ca2+ levels in hybrid white poplar (Populus alba x P. glandulosa) and examine the role of calcium signaling during the early stages of gravitropic responses. Our findings revealed differential cytosolic Ca2+ signal distribution in gravistimulated stems during the early phase of gravity induction, characterized by lower Ca2+ levels on the upper side (where TW forms) and higher Ca2+ levels on the lower side (where opposite wood forms). Consistent with this hypothesis, plants treated with LaCl3 and those with genetically disrupted calcium channels (PagGLR3.3 knockout using the CRISPR/Cas9 system) showed reduced Ca2+ signals and developed characteristic TW features. These results suggest that decreased Ca2+ levels induce the formation of TW. Furthermore, PagGLR3.3 knockout plants with TW-like stems displayed diminished sensitivity to gravistimulation. Transcriptomic analysis revealed that the knockout of PagGLR3.3 resulted in the upregulation of genes associated with TW formation and reactive oxygen species (ROS) production. Notably, superoxide anion (O2 -) levels were significantly elevated in the cambium zone of stems subjected to gravistimulation, LaCl3 treatment, or PagGLR3.3 knockout, indicating that reduced Ca2+ levels promote TW formation through increased O2 - accumulation. This study offers novel insights into the critical role of Ca2+ in gravitropism and TW induction in poplar.