文献类型：期刊文献

中文题名：棕榈藤基因组DNA提取及RAPD反应条件探索

英文题名：Genomic DNA Extraction and RAPD Protocols for Rattan

作者：李发根[1] 杨华[1] 尹光天[1] 许煌灿[1] 甘四明[1]

第一作者：李发根

机构：[1]中国林业科学研究院热带林业研究所

年份：2004

卷号：17

期号：6

起止页码：824-828

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家科技攻关计划(2001BA506b04 01)

语种：中文

中文关键词：棕榈藤;基因组;DNA;提取;RAPD;反应条件

外文关键词：rattan;genomic DNA extraction;CTAB;RAPD

分类号：Q949.715;S792.91

摘要：Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.
Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.