文献类型：期刊文献

英文题名：Rhizosphere microbiome drives Betula luminifera adaptation to antimony mining sites through functional traits and transcriptional reprogramming

作者：Xing, Wenli[1,2] Gai, Xu[1] Cheng, Xiangrong[1] Fang, Zitong[2] Chen, Guangcai[1]

第一作者：Xing, Wenli

通信作者：Chen, GC[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Xinjiang Univ, Coll Ecol & Environm, Urumqi 830017, Peoples R China

年份：2026

卷号：501

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20260219887186);Scopus(收录号：2-s2.0-105026857213);WOS:【SCI-EXPANDED(收录号:WOS:001658337800001)】；

基金：This work was supported by the National Key R & D Program of China (2020YFC1807704) , and Zhejiang provincial Ten thousand Talents Program for Leading Talents of Science and Technology Innovation (2021R52047) . We thank the Shi Zhou, Zhongyu Du and Wanqin Shu for assistance in plant detection, management and collecting these samples.

语种：英文

外文关键词：Sb mining sites; Transcriptome; Metagenome; Adaptability

摘要：Rhizosphere microbiome are pivotal for plant adaptation to extreme environments. However, the regulatory mechanisms underlying their control of the ecological adaptation of native woody plants in mining areas remain unclear. Here, we integrated metagenomic and transcriptomic analyses to elucidate how the rhizosphere microbiome facilitates Betula luminifera adaptation to antimony (Sb) mining sites. Under sterile conditions, B. luminifera from mining sites prioritized shoot growth, whereas control-origin seedlings favored root development. Microbial inoculation mitigated this growth dichotomy, balancing above- and belowground biomass allocation. Notably, B. luminifera from control sites upregulated antioxidant biosynthesis genes (alpha- and beta-tocopherol pathways), while B. luminifera from mining sites enhanced lignin synthesis under Sb stress. After inoculation with rhizosphere microbiome from the mining-site, genes related to Sb/As resistance (ACR3, arsB/C) and soil nutrient cycle (narG, phnM) were significantly enriched in the rhizosphere of B. luminifera, which were contributed by Proteobacteria and Actinobacteria. Transcriptional profiling revealed that microbial inoculation triggered systemic upregulation of phytohormone-related genes (auxin, cytokinin, abscisic acid), enhancing stress resilience and growth. These findings unveil a synergistic plant-microbe adaptation mechanism in Sb polluted soils in mining sites, highlighting microbial-mediated trait trade-offs and transcriptional plasticity as drivers of ecological success in extreme environments.