文献类型：期刊文献

英文题名：Seventeen novel microsatellite markers for a valuable medicinal plant Bletilla striata and cross-amplification in B-ochracea

作者：Wang, Caixia[1] Tian, Min[1]

第一作者：王彩霞

通信作者：Tian, M[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2015

卷号：7

期号：3

起止页码：715-716

外文期刊名：CONSERVATION GENETICS RESOURCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000359521500025)】；

基金：This work was supported by the cooperation project of Zhejiang province and Chinese academy of forestry for forestry science and technology (2014SY07).

语种：英文

外文关键词：Bletilla striata; Microsatellite; Marker; Bletilla ochracea

摘要：Bletilla striata, a valuable medicinal plant, has become rare and endangered due to limited natural reproduction, increased utilization and habitat destruction. Here, 17 polymorphic microsatellite markers were developed and characterized for B. striata. Per locus 3-16 alleles were detected with an average of 8.2. The polymorphic information content (PIC) varied from 0.146 to 0.895 with a mean of 0.607, and 14 loci had PIC > 0.5. The observed and expected heterozygosities ranged from 0.050 to 1.000 and from 0.050 to 0.901, respectively. Cross-amplification indicated 15 loci could be amplified in the congeneric species B. ochracea and 14 loci were polymorphic with a mean PIC of 0.512. These novel markers will facilitate the investigation of population genetics for B. striata and B. ochracea, which may also contribute to their management and conservation.