文献类型：期刊文献

中文题名：红树林湿地石油降解菌群的构建

英文题名：Construction of petroleum-degrading bacterial consortium from mangrove sediments

作者：李玫[1] 廖宝文[1] 陈玉军[1]

第一作者：李玫

机构：[1]中国林业科学研究院热带林业研究所

年份：2012

卷号：31

期号：5

起止页码：553-557

中文期刊名：生态科学

外文期刊名：Ecological Science

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：中央级公益性科研院所基本科研业务费专项资金(RITFYWZX201111)

语种：中文

中文关键词：红树林湿地;石油降解菌群构建;最佳投加配比

外文关键词：mangrove wetlands; petroleum-degrading bacterial consortium construction; optimum dosage ratio

分类号：S796;S714

摘要：近年来海洋污染正在日趋加剧,其中石油污染尤为严重。目前,采用微生物降解是解决海洋石油污染的有效途径之一,而滨海区域的红树林湿地是石油残留聚集和降解的重要生态系统之一。为了构建降解石油的优势菌群,分别以正十六烷烃和萘为唯一碳源,通过富集培养,从受石油污染的红树林淤泥(即土壤)中分离得到2株烷烃降解菌(即Z1、Z3)和2株芳香烃降解菌(即N1、N4)。采用三因素三水平进行正交试验,优化得到降解率最高的菌株组合,并确定了各菌株的最佳投加配比。结果表明:烷烃降解菌Z1、Z3和芳香烃降解菌N1组合的菌群降解石油效果最好,当石油初始质量浓度为2.0 g.L-1,接种量6%,30℃好氧培养72 h,石油降解率达47.3%;且当N1、Z1、Z3三种菌的投加配比为3:1:3时降解效果最佳,好氧培养72 h,石油降解率达51.2%。
In recent years,marine pollution has been increasing at global level,and the petroleum pollution is particularly serious.The technology of microbial degradation has become one of effective ways to solve marine petroleum pollution.Mangrove ecosystem plays an important role in accumulating and degrading petroleum residues in the coastal areas.In order to construct the petroleum-degrading bacterial consortium,with alkanes or naphthalene as the sole carbon source,two alkane degrading strains（named Z1,Z3） and two aromatic hydrocarbon-degrading strains（named N1,N4） were isolated by the enrichment culture method（ECM） from the petroleum contaminated mangrove sediments.Using the orthogonal experiments of three factors and three levels,the optimized strain combination was obtained,and the optimum dosages of each strain were determined.The results showed that a combination of two alkane-degrading strains Z1,Z3 and one aromatic-degrading strain N1 got the highest degradation rate of 47.3%,with the initial petroleum-hydrocarbon concentration of 2.0 g.L-1,the inoculation amount of 6%,the culture temperature of 30℃,and the aerobic culture time of 72 h.When N1,Z1 and Z3 dosing with the ratio of 3： 1： 3,the strain combination got the highest degradation rate of 51.2% after 72 h aerobic culture.