文献类型：期刊文献

中文题名：转双价抗蛀干害虫基因杨树的获得及其抗虫性鉴定

英文题名：Transformation of Poplar (Populus alba × P. glandulosa cv.`84K') with Binary Insect Resistant Genes and Analysis of Insect Resistance

作者：张冰玉[1] 苏晓华[1] 李义良[1] 张永安[2] 曲良建[2] 王玉珠[2] 田颖川[3]

第一作者：张冰玉

机构：[1]中国林业科学研究院林业研究所;[2]中国林业科学研究院森林生态与环境保护研究所;[3]中国科学院微生物研究所

年份：2005

卷号：18

期号：3

起止页码：364-368

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家转基因植物研究与产业化专项课题--优质高产抗干旱耐盐碱杨树基因工程育种研究(J2002 B 004)

语种：中文

中文关键词：杨树;遗传转化;双价基因;蛀干害虫;抗虫性

外文关键词：Populus; transformation; binary genes; coleopterous insect ; insect resistance

分类号：S792.11

摘要：Transgenic poplar(Populus alba×P.glandulosa cv`84k’) plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar’s toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.
Transgenic poplar(Populus alba×P.glandulosa cv`84k') plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar's toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.