文献类型：期刊文献

中文题名：多年生黑麦草愈伤组织航天搭载后再生株系生长性状的变异研究

英文题名：GROWTH TRAITS ANALYSIS OF REGENERATED PLANTS FROM CALLI OF Lolium perenne L. CARRIED BY SPACESHIP

作者：彭丽梅[1,2] 孙振元[2] 赵梁军[1] 韩蕾[2] 钱永强[2]

第一作者：彭丽梅

机构：[1]中国农业大学农学与生物技术学院;[2]中国林业科学研究院林业研究所/国家林业局林木重点实验室

年份：2011

卷号：25

期号：4

起止页码：645-651

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家"863"计划资助项目(2007AA100105;2009AA102109)

语种：中文

中文关键词：多年生黑麦草;空间诱变;体细胞无性系变异;生长性状;群体变异分析

外文关键词：Lolium perenne L.; space treatment; somaclonal variation; growth trait; group variation analysis

分类号：S688.4

摘要：将来自同一粒种子的多年生黑麦草(Lolium perenne L.)品种德比(Derby)的胚性愈伤组织利用神舟7号飞船搭载进行空间诱变处理,采用相同的诱导培养方式获得了搭载和未搭载愈伤组织2个再生株系群体。通过测定再生株系的叶片相对叶绿素含量(SPAD值)、叶片相对含水量、叶宽、单位叶面积鲜重及单位叶面积干重等生长指标,比较了2个再生株系群体的生长差异及其变异频率。结果表明,搭载再生株系(SP)群体各生长性状的变异系数均大于未搭载再生株系(NSP)群体。与NSP相比,SP叶片显著变窄(P<0.01),平均单位叶面积干重则显著增加(P<0.01);叶片SPAD值、叶片相对含水量下降,单位叶面积鲜重增加,但差异均不显著(P>0.05)。以超出均值的95%置信区间为筛选标准(xx珋+1.96S),从SP中筛选出叶宽、叶色、叶片相对含水量、单位叶面积鲜重及单位叶面积干重等性状发生变异的株系31个。认为空间诱变与植物组织培养相结合能有效提高诱变频率。
Embryogenic calli of Lolium perenne L.variety Derby from one seed were carried by Shenzhou No.7 spaceship for space treatment.Plants were regenerated from carried calli（SP） and uncarried calli（NSP）.The SPAD value（an index of relative chlorophyl（content））,relative water content（RWC）,leaf width,fresh weight per unit leaf area,and dry weight per unit leaf area were measured to compare the differences of growth traits and variation frequency between SP group and NSP group.The results showed that coefficients of variation in the growth traits of SP group were larger than those of NSP group.Leaf width of SP was significantly smaller than that of the NSP（P0.01）.The dry weight per unit leaf area of SP was significantly larger than that of NSP（P0.01）.Compared with NSP,the SPAD value and RWC of SP were reduced,and the fresh weight per unit leaf area was increased,but the differences weren＇t significant（P0.05）.31 mutants varied in leaf width,leaf color,relative water content,fresh weight per unit leaf area and dry weight per unit leaf area were selected from the SP with the standard of x＋1.96S,x＋1.96S.It is concluded that combination of the tissue culture technique and the spaceflight could increase the mutagenic frequency.