文献类型：期刊文献

中文题名：适于杨树功能基因组研究的T-DNA激活标签构建

英文题名：Construction of T-DNA Activation Tagging for Populus Functional Genomics

作者：王曙光[1] 栾维江[2] 乔桂荣[2] 孙宗修[3] 卓仁英[2]

第一作者：王曙光

机构：[1]西北农林科技大学;[2]中国林业科学研究院亚热带林业研究所;[3]中国水稻研究所水稻生物学国家重点实验室

年份：2007

卷号：20

期号：4

起止页码：586-590

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家林业局"948"项目"安全选择标记基因及林木转基因新技术引进"(2005-4-58)部分内容

语种：中文

中文关键词：杨树;T-DNA激活标签;功能基因组学;bar基因

外文关键词：Populus ; T-DNA activation tagging; functional genomics ; bar gene

分类号：Q78

摘要：With the completion of the Populus genome sequence in 2006,the study on functional genomics in Populus has become a major task.Establishment of Populus mutant population is an essential approach for forestry functional genomics study.The activation tagging is a promising and powerful method to construct mutant library of Populus and to further discover new gene and elucidate its function now.A novel T-DNA activation tagging pCAS05 was constructed for forest functional genomics in this study.We could select conveniently and easily transgenic plants by spraying Basta because bar gene is constructed in this vector as a selection marker.Moreover,we could also construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bar gene.
With the completion of the Populus genome sequence in 2006, the study on functional genomics in Populus has become a major task. Establishment of Populus mutant population is an essential approach for forestry functional genomics study. The activation tagging is a promising and powerful method to construct mutant library of Pop- ulus and to further discover new gene and elucidate its function now. A novel T-DNA activation tagging pCAS05 was constructed for forest functional genomics in this study. We could select conveniently and easily transgenic plants by spraying Basta because bar gene is constructed in this vector as a selection marker. Moreover, we could also construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bar gene.