文献类型：期刊文献

英文题名：Identification and Characterization of LaSCL6 Alleles in Larix kaempferi (Lamb.) Carr. Based on Analysis of Simple Sequence Repeats and Allelic Expression

作者：Zang, Qiao-Lu[1] Li, Xiang-Yi[1] Qi, Li-Wang[1] Li, Wan-Feng[1]

第一作者：Zang, Qiao-Lu

通信作者：Li, WF[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：11

期号：12

起止页码：1-10

外文期刊名：FORESTS

收录：;EI(收录号：20204909595873);Scopus(收录号：2-s2.0-85097129043);WOS:【SCI-EXPANDED(收录号:WOS:000601994300001)】；

基金：This work was supported by the National Natural Science Foundation of China (31770714), the Basic Research Fund of Research Institute of Forestry (RIF2014-07), and the National Transgenic Major Program (2018ZX08020-003).

语种：英文

外文关键词：Japanese larch; endosperm; haplotypes; genetics; breeding

摘要：Simple sequence repeats (SSRs) are widely used as markers for the assessment of genetic diversity and marker-assisted breeding. In a previous study, two SSRs (GCA and CCA), were found in the genomic sequence of Larix (La) SCL6, which plays important roles in the growth and development of Larix kaempferi (Lamb.) Carr. In this study, we analyzed the polymorphisms of these two SSRs in the L. kaempferi population. We found that each SSR had five different polymorphisms, among which (GCA)(7) and (CCA)(7) were predominant. In addition, 12 haplotypes were detected, with (GCA)(7)(CCA)(7) having the highest frequency. Furthermore, we detected the haplotypes of LaSCL6 in mature trees and their seeds and analyzed the relationships between parents and offspring. The expression patterns of five LaSCL6 alleles were analyzed and they showed balanced expression during vegetative development. Taken together, these findings not only provide more genetic information on LaSCL6, but also provide a candidate marker for genetic studies and breeding.